|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Immunogen||The details of the immunogen for this antibody are not currently available.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:100|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes a non-polymorphic epitope on the mouse CD8 alpha chain. It blocks MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Two alternative transcripts encoding distinct isoforms, one membrane associated and one secreted, have been identified [Gene Wiki: CD8a].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Fowlpox-based survivin vaccination for malignant mesothelioma therapy.
MA5-17006 was used in immunohistochemistry to study the therapeutic potential of a novel Fowlpox-based survivin vaccination in a murine model of malignant mesothelioma
|Bertino P,Panigada M,Soprana E,Bianchi V,Bertilaccio S,Sanvito F,Rose AH,Yang H,Gaudino G,Hoffmann PR,Siccardi A,Carbone M||International journal of cancer (133:612)||2013|
Consequences of the combined loss of BOK and BAK or BOK and BAX.
MA5-17006 was used in flow cytometry to determine if Bok and Bax have any functional redundancy controlling apoptosis using knock out mice.
|Ke F,Bouillet P,Kaufmann T,Strasser A,Kerr J,Voss AK||Cell death and disease (4:null)||2013|
Development of thymic microenvironments in vitro is oxygen-dependent and requires permanent presence of T-cell progenitors.
MA5-17006 was used in flow cytometry to investigate factors that mediate induction and maintenance of the 3D configuration of the epithelial network in fetal thymic lobes in vitro.
|Germeraad WT,Kawamoto H,Itoi M,Jiang Y,Amagai T,Katsura Y,van Ewijk W||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (51:1225)||2003|
Commitment of common T/Natural killer (NK) progenitors to unipotent T and NK progenitors in the murine fetal thymus revealed by a single progenitor assay.
MA5-17006 was used in flow cytometry to describe a clonal system that supports the development of T and natural killer cells evenly.
|Ikawa T,Kawamoto H,Fujimoto S,Katsura Y||The Journal of experimental medicine (190:1617)||1999|
Primed T cells are more resistant to Fas-mediated activation-induced cell death than naive T cells.
MA5-17006 was used in flow cytometry to study Fas-mediated activation-induced cell death in naive and primed T cells.
|Inaba M,Kurasawa K,Mamura M,Kumano K,Saito Y,Iwamoto I||Journal of immunology (Baltimore, Md. : 1950) (163:1315)||1999|
Effects of dietary docosahexaenoic acid on surface molecules involved in T cell proliferation.
MA5-17006 was used in flow cytometry to examine the effect of dietary docosahexaenoic acid on the surface molecules involved in T cell proliferation.
|Sasaki T,Kanke Y,Kudoh K,Misawa Y,Shimizu J,Takita T||Biochimica et biophysica acta (1436:519)||1999|
A vascular smooth muscle-specific CD4+ T cell line that induces pulmonary vasculitis in MRL-+/+ mice.
MA5-17006 was used in flow cytometry to develop and characterize a MV1-induced vasculitis model.
|Sumita S,Ozaki S,Okazaki T,Sobajima J,Nakao K||Clinical and experimental immunology (105:163)||1996|
Exclusive development of T cell neoplasms in mice transplanted with bone marrow expressing activated Notch alleles.
MA5-17006 was used in flow cytometry to report that TAN1 is an oncoprotein.
|Pear WS,Aster JC,Scott ML,Hasserjian RP,Soffer B,Sklar J,Baltimore D||The Journal of experimental medicine (183:2283)||1996|
Decreased CD4-CD8- TCR-alpha beta + cells in lpr/lpr mice lacking beta 2-microglobulin.
MA5-17006 was used in flow cytometry to test the requirement for CD8 to interact with class I MHC molecules to promote CD4-CD8- T cells in lpr/lpr mice.
|Mixter PF,Russell JQ,Durie FH,Budd RC||Journal of immunology (Baltimore, Md. : 1950) (154:2063)||1995|
In vivo CD4+ lymph node T cells from lpr mice generate CD4-CD8-B220+TCR-beta low cells.
MA5-17006 was used in flow cytometry to use an in vivo transfer system to analyze the progeny of CD4+ T cells from B6-lpr/lpr mice.
|Laouar Y,Ezine S||Journal of immunology (Baltimore, Md. : 1950) (153:3948)||1994|
CD3+ and CD4+ cells adoptively transfer experimental hypersensitivity pneumonitis.
MA5-17006 was used in flow cytometry to describe the cells that transfer adoptive murine experimental hypersensitivity pneumonitis.
|Schuyler M,Gott K,Shopp G,Crooks L||The American review of respiratory disease (146:1582)||1992|
The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells.
MA5-17006 was used in flow cytometry to develop a new method to isolate dendritic cells and characterize splenic and thymic dendritic cells.
|Vremec D,Zorbas M,Scollay R,Saunders DJ,Ardavin CF,Wu L,Shortman K||The Journal of experimental medicine (176:47)||1992|
|Not Applicable||Not Cited||
The earliest thymic progenitors in adults are restricted to T, NK, and dendritic cell lineage and have a potential to form more diverse TCRbeta chains than fetal progenitors.
MA5-17006 was used in flow cytometry to study T cell progenitors in the adult thymus
|Lu M,Tayu R,Ikawa T,Masuda K,Matsumoto I,Mugishima H,Kawamoto H,Katsura Y||Journal of immunology (Baltimore, Md. : 1950) (175:5848)||2005|