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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1.0 ug/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|T-Cell Activation (TCA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA5-17594 targets CD8a/T subset NK in FACS, IHC (F), IHC (P), and TCA applications and shows reactivity with Mouse samples.
The MA5-17594 immunogen is mouse CD8a.
This antibody recognizes CD8. CD8 is composed of an alpha chain with Mr=30-34 kDa linked by disulfide bonds to a beta chain with Mr=30-32 kDa. In addition to forming heterodimers with the beta chain, the alpha chain is also capable of forming homodimers while the beta chain is incapable of forming homodimers. The extracellular portion of CD8alpha binds to the non-polymorphic portion of MHC class I molecules. This interaction is important in the antigen presentation to CD8+ T cells. The cytoplasmic domain of the alpha chain associates with p56lck, a member of the Src family of tyrosine kinases. Cells which are reactive with this antibody include: thymocytes, natural killer cells, and a subset (CD4-) of peripheral T-cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Progression of T cell lineage restriction in the earliest subpopulation of murine adult thymus visualized by the expression of lck proximal promoter activity.
MA5-17594 was used in flow cytometry to study regulation of the lck in T cell lineage commitment.
|Shimizu C,Kawamoto H,Yamashita M,Kimura M,Kondou E,Kaneko Y,Okada S,Tokuhisa T,Yokoyama M,Taniguchi M,Katsura Y,Nakayama T||International immunology (13:105)||2001|
CD8; CD8a; Leu-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); MAL; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2
BB154331; Cd8a; Ly-2; Ly-35; Ly-B; Lyt-2; Lyt2