|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 ug/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
MA5-17603 targets CD8a/T subset NK in FACS and IHC (F) applications and shows reactivity with Mouse samples.
The MA5-17603 immunogen is mouse thymocytes.
This antibody recognizes CD8. CD8 is composed of an alpha chain with Mr=30-34 kDa linked by disulfide bonds to a beta chain with Mr=30-32 kDa. In addition to forming heterodimers with the beta chain, the alpha chain is also capable of forming homodimers while the beta chain is incapable of forming homodimers. The extracellular portion of CD8alpha binds to the non-polymorphic portion of MHC class I molecules. This interaction is important in the antigen presentation to CD8+ T cells. The cytoplasmic domain of the alpha chain associates with p56lck, a member of the Src family of tyrosine kinases. Cells which are reactive with this antibody include: thymocytes, natural killer cells, and a subset (CD4-) of peripheral T-cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Candida albicans infection enhances immunosuppression induced by cyclophosphamide by selective priming of suppressive myeloid progenitors for NO production.
MA5-17603 was used in flow cytometry to examine the T cell responses in cyclophosphamide-treated mice after systemic infection with Candida albicans.
|Angulo I,Jiménez-Díaz MB,García-Bustos JF,Gargallo D,de las Heras FG,Muñoz-Fernández MA,Fresno M||Cellular immunology (218:46)||2002|
Surface antigen expression in spleen cells of C57B1/6 mice during ageing: influence of sex and parity.
MA5-17603 was used in flow cytometry to test if pregnancies and gender influence the cellar changes observed during ageing in mice.
|Barrat F,Lesourd BM,Louise A,Boulouis HJ,Vincent-Naulleau S,Thibault D,Sanaa M,Neway T,Pilet CH||Clinical and experimental immunology (107:593)||1997|