Abnova

CD8A Monoclonal Antibody (4B9)

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Protocols

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CD8A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence of monoclonal antibody to CD8A on HeLa cell. Antibody concentration is 40 µg/mL.

CD8A Antibody in Immunoprecipitation (IP) — Immunoprecipitation of CD8A transfected lysate using anti-CD8A monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CD8A MaxPab rabbit polyclonal antibody.

CD8A Antibody in ELISA (ELISA) — Detection limit for recombinant GST tagged CD8A is approximately 3 ng/mL as a capture antibody.

Product Details

H00000925-M10

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	40 µg/mL	-
ELISA (ELISA)	3 ng/mL	-
Immunoprecipitation (IP)	1-5 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2b, kappa
Class	Monoclonal
Type	Antibody
Clone	4B9
Immunogen	CD8A (AAH25715, 1 a.a. approximately 235 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate	Unconjugated
Form	Liquid
Concentration	See Label
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Sequence of this protein is as follows: MALPVTALLL PLALLLHAAR PSQFRVSPLD RTWNLGETVE LKCQVLLSNP TSGCSWLFQP RGAAASPTFL LYLSQNKPKA AEGLDTQRFS GKRLGDTFVL TLSDFRRENE GCYFCSALSN SIMYFSHFVP VFLPAKPTTT PAPRPPTPAP TIASQPLSLR PEACRPAAGG AVHTRGLDFA CDIYIWAPLA GTCGVLLLSL VITLYCNHRN RRRVCKCPRP VVKSGDKPSL SARYV

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

UniProt ID: (Human) P01732, (Human) P10966

Entrez Gene ID: (Human) 925, (Human) 926

Molecular Function: defense/immunity protein immunoglobulin receptor superfamily