|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1.0 ug/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 8 publications below|
MA5-17746 targets CD90/Thy-1 in FACS and IHC (F) applications and shows reactivity with Mouse samples.
The MA5-17746 immunogen is cBA/J.
Thy-1 or CD90 is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Stepwise development of committed progenitors in the bone marrow that generate functional T cells in the absence of the thymus.
MA5-17746 was used to conclude that bone marrow committed T cell progenitors can generate functional T cells via an extrathymic pathway
|García-Ojeda ME,Dejbakhsh-Jones S,Chatterjea-Matthes D,Mukhopadhyay A,BitMansour A,Weissman IL,Brown JM,Strober S||Journal of immunology (Baltimore, Md. : 1950) (175:4363)||2005|
Early defect prethymic in bone marrow T cell progenitors in athymic nu/nu mice.
MA5-17746 was used in flow cytometry to examine early T cell progenitor development in the nu/nu bone marrow.
|Chatterjea-Matthes D,García-Ojeda ME,Dejbakhsh-Jones S,Jerabek L,Manz MG,Weissman IL,Strober S||Journal of immunology (Baltimore, Md. : 1950) (171:1207)||2003|
Donor-type CD4(+)CD25(+) regulatory T cells suppress lethal acute graft-versus-host disease after allogeneic bone marrow transplantation.
MA5-17746 was used in flow cytometry to report that the balance of donor-type CD4(+)CD25(+) T(reg) and conventional CD4(+)CD25(-) T cells determines the outcome of acute graft-versus-host disease.
|Hoffmann P,Ermann J,Edinger M,Fathman CG,Strober S||The Journal of experimental medicine (196:389)||2002|
Predominance of NK1.1+TCR alpha beta+ or DX5+TCR alpha beta+ T cells in mice conditioned with fractionated lymphoid irradiation protects against graft-versus-host disease: "natural suppressor" cells.
MA5-17746 was used in flow cytometry to develop a nonmyeloablative host conditioning regimen for transplantation.
|Lan F,Zeng D,Higuchi M,Huie P,Higgins JP,Strober S||Journal of immunology (Baltimore, Md. : 1950) (167:2087)||2001|
Clonable progenitors committed to the T lymphocyte lineage in the mouse bone marrow; use of an extrathymic pathway.
MA5-17746 was used in flow cytometry to identify progenitors that can result in T cells via an extrathymic pathway.
|Dejbakhsh-Jones S,Garcia-Ojeda ME,Chatterjea-Matthes D,Zeng D,Strober S||Proceedings of the National Academy of Sciences of the United States of America (98:7455)||2001|
Allogeneic bone marrow cells that facilitate complete chimerism and eliminate tumor cells express both CD8 and T-cell antigen receptor-alphabeta.
MA5-17746 was used in flow cytometry to test if specific subsets of cells in allogeneic bone marrow transplants can treat the BCL(1) B-cell lymphoma without inducing graft verses host disease.
|Lan F,Zeng D,Huie P,Higgins JP,Strober S||Blood (97:3458)||2001|
Identification of an early T cell progenitor for a pathway of T cell maturation in the bone marrow.
MA5-17746 was used in flow cytometry to identify a rare population of cells in the adult mouse bone marrow that generates CD4(+) and CD8(+) TCRalphabeta(+) T cells.
|Dejbakhsh-Jones S,Strober S||Proceedings of the National Academy of Sciences of the United States of America (96:14493)||1999|
Effects of the lpr mutation on elimination and inactivation of self-reactive B cells.
MA5-17746 was used in flow cytometry to investigate the fate of autoreactive B cells in lpr/lpr mice.
|Rathmell JC,Goodnow CC||Journal of immunology (Baltimore, Md. : 1950) (153:2831)||1994|