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Invitrogen
CDK9 Monoclonal Antibody (K.513.1)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Product Details
MA5-14912
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Hamster,
Human,
Mouse,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
K.513.1
Immunogen
Synthetic peptide corresponding to residues near the carboxy terminus of human CDK9
Conjugate
Unconjugated
Form
Liquid
Concentration
163 µg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
RRID
AB_10986858
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
CDK9 (cyclin-dependent kinase 9) is a member of the cyclin-dependent protein kinase family. It is involved in the regulation of transcription. CDK9 is a member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex. This complex facilitates the transition from abortive to productive elongation by phosphorylating the CTD of the large subunit of RNA polymerase II, POLR2A, SUPT5H, and RDBP. The complex is inactive when in the 7SK snRNP complex form. CDK9 phosphorylates EP300, MYOD1, RPB1/POLR2A, AR, DSIF, and NELF. It regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and confers a predisposition to heart failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-2K; CDC2-related kinase; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Cyclin-dependent kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); RP11-228B15.5; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit
Gene Aliases: C-2k; CDC2L4; CDK9; CTK1; PITALRE; TAK
UniProt ID: (Human) P50750, (Bovine) Q5EAB2, (Mouse) Q99J95, (Rat) Q641Z4
Entrez Gene ID: (Human) 1025, (Bovine) 520580, (Dog) 100855805, (Chinese hamster) 100757719, (Mouse) 107951, (Rat) 362110
Molecular Function:
non-receptor serine/threonine protein kinase
DNA repair
regulation of DNA repair
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
protein phosphorylation
regulation of mitotic cell cycle
cell proliferation
positive regulation of cardiac muscle hypertrophy
regulation of histone modification
replication fork processing
positive regulation of histone phosphorylation
response to drug
snRNA transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral transcription
regulation of muscle cell differentiation
negative regulation of cell cycle arrest
cellular response to cytokine stimulus
negative regulation of mRNA polyadenylation
positive regulation of mRNA 3'-UTR binding
positive regulation of histone H2B ubiquitination
phosphorylation of RNA polymerase II C-terminal domain
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
phosphorylation
replication fork arrest
RNA polymerase II core promoter sequence-specific DNA binding
transcription coactivator binding
DNA binding
chromatin binding
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
7SK snRNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
protein kinase binding
nucleotide binding
transcription factor binding
transferase activity
transferase activity, transferring phosphorus-containing groups
snRNA binding
transcription regulatory region DNA binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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