Immunofluorescent detection of p19Arf. Wild type mouse embryo fibroblasts (MEF) at passage 5 (panels 1, 2) and NIH3T3 cells, which have deleted the Arf gene (panels 3, 4) were probed with MA1-16665, followed by a fluorescently labeled anti-rat IgG secondary antibody (panels 1, 3). Cells were also stained with Hoechst dye to reveal the position of nuclei (panels 2, 4).
|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Synthetic peptide containing amino acids 54-75 of the murine p19ARF.|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:1000|
|Immunohistochemistry (IHC)||5 µ/gml|
|Immunohistochemistry (Frozen) (IHC (F))||5 µg/ml|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody does not react with human or Golden hamster.
Armenian hamster has not been tested.
In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.