|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||His-tagged recombinant full length p15 protein.|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
The four known 15-to 19 kDa INK4 proteins (p15INK4b, p16INK4a, p18INK4c andp19INK4d) bind and inhibit CDK4 and CDK6 but not other CDKs, including cyclin E-Cdk2, cyclin A-Cdk2 and cyclin B-Cdk1. Like the three D-type cyclins, the INK4 genes are expressed in distinct tissue-specific patterns, suggesting that they are not strictly redundant. 5 p18INK4c is expressed during G1 to S transition in the eukaryotic cell division cycle. It is maximally induced as cells enter S-phase. The availability of a monoclonal antibody reacting specifically with p18INK4c enables the subcellular detection and localization of p18INK4c and the measurement of relative differences in p18INK4c levels as a function of cell cycle phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification of Cyclobutane Pyrimidine Dimer-Responsive Genes Using UVB-Irradiated Human Keratinocytes Transfected with In Vitro-Synthesized Photolyase mRNA.
MA1-12294 was used in western blot to investigate the contribution of cyclobutane pyrimidine dimers to UVB-induced changes of gene expression.
|Boros G,Miko E,Muramatsu H,Weissman D,Emri E,van der Horst GT,Szegedi A,Horkay I,Emri G,Karikó K,Remenyik É||PloS one (10:null)||2015|