Immunohistochemistry analysis of COX2 showing staining in the cytoplasm and membrane of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a COX2 monoclonal antibody (Product # MA5-14568) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Dog, Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from C-terminus of rat cox2|
|Storage buffer||tissue culture supernatant|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1:200 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14568 targets COX2 in IHC (P) applications and shows reactivity with Human samples.
The MA5-14568 immunogen is a synthetic peptide from C-terminus of rat cox2.
COX2 (Cyclooxygenase-2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate-early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Loss of Cdh1 and Trp53 in the uterus induces chronic inflammation with modification of tumor microenvironment.
MA5-14568 was used in immunohistochemistry - paraffin section to determine how loss of Trp53 and Cdh1 in the uterus can induce chronic inflammation with a change in the tumor microenvironment
|Stodden GR,Lindberg ME,King ML,Paquet M,MacLean JA,Mann JL,DeMayo FJ,Lydon JP,Hayashi K||Oncogene (34:2471)||2015|
Systems biology analysis reveals NFAT5 as a novel biomarker and master regulator of inflammatory breast cancer.
MA5-14568 was used in immunohistochemistry - paraffin section to identify master regulators of inflammatory breast cancer
|Remo A,Simeone I,Pancione M,Parcesepe P,Finetti P,Cerulo L,Bensmail H,Birnbaum D,Van Laere SJ,Colantuoni V,Bonetti F,Bertucci F,Manfrin E,Ceccarelli M||Journal of translational medicine (13:null)||2015|
|Not Applicable||Not Cited||
Early degenerative effects of diabetes mellitus on pancreas, liver, and kidney in rats: an immunohistochemical study.
MA5-14568 was used in immunohistochemistry - paraffin section to perform an immunohistochemical study and early degenerative effects of diabetes mellitus on liver, kidney and pancreas in rats
|Haligur M,Topsakal S,Ozmen O||Experimental diabetes research (2012:null)||2012|
|Not Applicable||Not Cited||
Regional Differences in the Neuronal Expression of Cyclooxygenase-2 (COX-2) in the Newborn Pig Brain.
MA5-14568 was used in immunohistochemistry - paraffin section to study neuronal Cox-2 expression in different regions of the newborn pig brain
|Oláh O,Németh I,Tóth-Sz¿ki V,Bari F,Domoki F||Acta histochemica et cytochemica (45:187)||2012|
Markers aiding the diagnosis of chondroid tumors: an immunohistochemical study including osteonectin, bcl-2, cox-2, actin, calponin, D2-40 (podoplanin), mdm-2, CD117 (c-kit), and YKL-40.
MA5-14568 was used in immunohistochemistry - paraffin section to identify immunohistochemical markers to aid chondroid tumor diagnosis
|Daugaard S,Christensen LH,Høgdall E||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (117:518)||2009|
An immunohistochemical study of feline endometrial adenocarcinoma.
MA5-14568 was used in immunohistochemistry - paraffin section to characterize feline endometrial adenocarcinomas immunohistochemically
|Gil da Costa RM,Santos M,Amorim I,Lopes C,Pereira PD,Faustino AM||Journal of comparative pathology (140:254)||2009|
Stromal, rather than epithelial cyclooxygenase-2 (COX-2) expression is associated with overall survival of breast cancer patients.
MA5-14568 was used in immunohistochemistry to discuss the relevance of COX-2 expression in breast cancer
|Urban J,Ku¿bicki ¿,Szatkowski G,Stanek-Widera A,Lange D,Chwirot BW||BMC cancer (14:null)||2014|
Effects of maternally exposed food coloring additives on laryngeal histology in rats.
MA5-14568 was used in immunohistochemistry to study the effect of artificial food colors and additives on laryngeal histomorphology and immunohistochemical expression in maternally exposed rats.
|Ba¿ak K,Doguç DK,Aylak F,Karadayi N,Gültekin F||Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer (33:123)||2014|
Predictive utility of cyclo-oxygenase-2 expression by colon and rectal cancer.
MA5-14568 was used in immunohistochemistry to study the value of COX-2 expression in predicting disease-specific and disease-free survival in colon and rectal cancers
|Lobo Prabhu KC,Vu L,Chan SK,Phang T,Gown A,Jones SJ,Wiseman SM||American journal of surgery (207:712)||2014|
Analysis of a synchronous gliosarcoma and meningioma with long survival: A case report and review of the literature.
MA5-14568 was used in immunohistochemistry to report on a case of synchronous gliosarcoma and meningioma
|Linhares P,Martinho O,Carvalho B,Castro L,Lopes JM,Vaz R,Reis RM||Surgical neurology international (4:null)||2014|
Endothelial cell whole genome expression analysis in a mouse model of early-onset Fuchs' endothelial corneal dystrophy.
MA5-14568 was used in immunohistochemistry to study the gene expression profile of endothelial cells in a murine model of early-onset Fuch's endothelial corneal dystrophy
|Matthaei M,Hu J,Meng H,Lackner EM,Eberhart CG,Qian J,Hao H,Jun AS||Investigative ophthalmology and visual science (54:1931)||2013|
Combination of molecular alterations and smoking intensity predicts bladder cancer outcome: a report from the Los Angeles Cancer Surveillance Program.
MA5-14568 was used in immunohistochemistry to study the value of a panel of 9 biomarkers plus smoking intensity in predicting the outcome of bladder cancer
|Mitra AP,Castelao JE,Hawes D,Tsao-Wei DD,Jiang X,Shi SR,Datar RH,Skinner EC,Stein JP,Groshen S,Yu MC,Ross RK,Skinner DG,Cortessis VK,Cote RJ||Cancer (119:756)||2013|
Expression of Ki67, BCL-2, and COX-2 in canine cutaneous mast cell tumors: association with grading and prognosis.
MA5-14568 was used in immunohistochemistry to study the pathological and prognostic value of Ki67, BCL-2 and COX-2 expression in canine cutaneous mast cell tumors
|Vascellari M,Giantin M,Capello K,Carminato A,Morello EM,Vercelli A,Granato A,Buracco P,Dacasto M,Mutinelli F||Veterinary pathology (50:110)||2013|
Assessment of COX-2 expression presence and severity by immunohistochemical method in patients with chronic active gastritis and intestinal metaplasia.
MA5-14568 was used in immunohistochemistry to examine patients with either chronic active gastitis or intestinal metaplasia for the immunohistochemical expression of COX-2
|Erkan G,Gönül ¿I,Kand¿lc¿ U,Dursun A||The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology (23:478)||2012|
|Not Applicable||Not Cited||
Cyclooxygenase-2 expression in bladder cancer and patient prognosis: results from a large clinical cohort and meta-analysis.
MA5-14568 was used in immunohistochemistry to determine the prognostic potential of COX2 expression in urothelial carcinoma of the bladder
|Czachorowski MJ,Amaral AF,Montes-Moreno S,Lloreta J,Carrato A,Tardón A,Morente MM,Kogevinas M,Real FX,Malats N||PloS one (7:null)||2012|
Inflammatory myofibroblastic tumor of the mesentery: a clinical dilemma.
MA5-14568 was used in immunohistochemistry to report on a case of inflammatory myofibroblastic tumor
|Shatzel J,Wooten K,Ankola A,Cheney RT,Morrison CD,Skitzki JJ||International journal of clinical oncology (17:380)||2012|
Evaluation of cyclooxygenase-2 expression in canine mast cell tumours.
MA5-14568 was used in immunohistochemistry to investigate the expression of cyclooxygenase-2 in canine mast cell tumors
|Prada J,Queiroga FL,Gregório H,Pires I||Journal of comparative pathology (147:31)||2012|
Immunoexpression of cyclooxygenase-2 in primary gastric carcinomas and lymph node metastases.
MA5-14568 was used in immunohistochemistry to study the immunohistochemical expression of COX-2 in primary gastric carcinomas and lymph node metastases
|Almeida PR,Ferreira FV,Santos CC,Rocha-Filho FD,Feitosa RR,Falcão EA,Cavada BK,Lima-Júnior RC,Ribeiro RA||World journal of gastroenterology (18:778)||2012|
Intense CD44 expression is a negative prognostic factor in tonsillar and base of tongue cancer.
MA5-14568 was used in immunohistochemistry to study the prognostic value of the immunohistochemical expression of CD44 in patients with tonsillar and base of tongue cancer
|Lindquist D,Ahrlund-Richter A,Tarján M,Tot T,Dalianis T||Anticancer research (32:153)||2012|
COX-2 expression predicts worse breast cancer prognosis and does not modify the association with aspirin.
MA5-14568 was used in immunohistochemistry to identify the relationship between COX-2 expression and improved cancer survival with aspirin
|Holmes MD,Chen WY,Schnitt SJ,Collins L,Colditz GA,Hankinson SE,Tamimi RM||Breast cancer research and treatment (130:657)||2011|
Immunohistochemical COX-2 overexpression correlates with HER-2/neu overexpression in invasive breast carcinomas: a pilot study.
MA5-14568 was used in immunohistochemistry to investigate the correlation of COX-2 overexpression with HER-2/neu overexpression in invasive breast carcinomas
|Çiri¿ IM,Bozkurt KK,Ba¿pinar S,Kapucuo¿lu FN||Pathology, research and practice (207:182)||2011|
Overexpression of cyclooxygenase-2 as a biomarker in different subtypes of the oral lichenoid disease.
MA5-14568 was used in immunohistochemistry to investigate the prognostic value of cyclooxygenase-2 in subtypes of the oral lichenoid disease
|Cortés-Ramírez DA,Rodríguez-Tojo MJ,Gainza-Cirauqui ML,Martínez-Conde R,Aguirre-Urizar JM||Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics (110:738)||2010|
The selective COX-2 inhibitor Etoricoxib reduces acute inflammatory markers in a model of neurogenic laryngitis but loses its efficacy with prolonged treatment.
MA5-14568 was used in immunohistochemistry to investigate the efficacy of Etoricoxib in treatment for neurogenic laryngitis
|Lima-Rodrigues M,Lamas N,Valle-Fernandes A,Cruz A,Vieira A,Oliveira P,Pedrosa J,Castro AG,Reis RM,Baltazar F,Almeida A||Inflammation research : official journal of the European Histamine Research Society ... [et al.] (59:743)||2010|
Cyclo-oxygenase-2 expression in photon-radiated and non-radiated uveal melanomas.
MA5-14568 was used in immunohistochemistry to investigate the expression level of COX-2 in uveal melanomas
|Suesskind D,Paulsen F,Buchgeister M,Spitzer B,Rohrbach JM,Bartz-Schmidt KU,Spitzer MS||Acta ophthalmologica (88:582)||2010|
Cyclooxygenase-2 expression and connection with tumor recurrence and histopathologic parameters in gastrointestinal stromal tumors.
MA5-14568 was used in immunohistochemistry to study the cyclooxygenase-2 expression and its effect on mesenchymal tumor growth and recurrence
|Türköz HK,Alkan I,Si¿man S,Ozcan D||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (117:825)||2009|
Cyclin D1 overexpression increases susceptibility to 4-nitroquinoline-1-oxide-induced dysplasia and neoplasia in murine squamous oral epithelium.
MA5-14568 was used in immunohistochemistry to investigate the effect of Cox-2 on premalignant and malignant oral epithelial lesions
|Wilkey JF,Buchberger G,Saucier K,Patel SM,Eisenberg E,Nakagawa H,Michaylira CZ,Rustgi AK,Mallya SM||Molecular carcinogenesis (48:853)||2009|
The effect of angiotensin II on the number of macula densa cells through the AT1 receptor.
MA5-14568 was used in immunohistochemistry to investigate the influence of angiotensin II on macula densa cells and its mechanism
|Razga Z,Nyengaard JR||Nephron. Physiology (112:p37)||2009|
Combination of an EGFR blocker and a COX-2 inhibitor for the treatment of advanced cutaneous squamous cell carcinoma.
MA5-14568 was used in immunohistochemistry to study the treatment of advanced cutaneous squamous cell carcinoma with combination of an EGFR blocker and a COX-2 inhibitor
|Jalili A,Pinc A,Pieczkowski F,Karlhofer FM,Stingl G,Wagner SN||Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG (6:1066)||2008|
Transgenic cyclooxygenase-2 expression and high salt enhanced susceptibility to chemical-induced gastric cancer development in mice.
MA5-14568 was used in immunohistochemistry to investigate the effect of high salt and COX-2 overexpression on chemical-induced gastric carcinogenesis
|Leung WK,Wu KC,Wong CY,Cheng AS,Ching AK,Chan AW,Chong WW,Go MY,Yu J,To KF,Wang X,Chui YL,Fan DM,Sung JJ||Carcinogenesis (29:1648)||2008|
Eicosanoid modulation in advanced lung cancer: cyclooxygenase-2 expression is a positive predictive factor for celecoxib + chemotherapy--Cancer and Leukemia Group B Trial 30203.
MA5-14568 was used in immunohistochemistry to investigate the eicosanoid-based therapy against advanced lung cancer
|Edelman MJ,Watson D,Wang X,Morrison C,Kratzke RA,Jewell S,Hodgson L,Mauer AM,Gajra A,Masters GA,Bedor M,Vokes EE,Green MJ||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (26:848)||2008|
A new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profile.
MA5-14568 was used in immunohistochemistry to determine the changes of COX-2 and cytokine expression in the laryngeal mucosa of the NGI model
|Lima-Rodrigues M,Valle-Fernandes A,Lamas N,Cruz A,Baltazar F,Milanezi F,Nunes R,Reis RM,Pedrosa J,Castro AG,Almeida A||The Laryngoscope (118:78)||2008|
Computer-assisted immunohistochemical analysis of cervical cancer biomarkers using low-cost and simple software.
MA5-14568 was used in immunohistochemistry to investigate the efficiency of specific software for cervical cancer immunohistochemistry data analysis
|Hammes LS,Korte JE,Tekmal RR,Naud P,Edelweiss MI,Valente PT,Longatto-Filho A,Kirma N,Cunha-Filho JS||Applied immunohistochemistry and molecular morphology : AIMM (15:456)||2007|
DNA content, Cyclooxygenase-2 expression and loss of E-cadherin expression do not predict risk of malignant transformation in oral lichen planus.
MA5-14568 was used in immunohistochemistry to evaluate DNA content, cyclooxygenase-2 expression and loss of E-cadherin as the markers for malignant transformation in oral lichen planus
|Neppelberg E,Johannessen AC||European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery (264:1223)||2007|
Delayed progression of pancreatic intraepithelial neoplasia in a conditional Kras(G12D) mouse model by a selective cyclooxygenase-2 inhibitor.
MA5-14568 was used in immunohistochemistry to investigate the effect of a selective cyclooxygenase-2 inhibitor on the progression of pancreatic intraepithelial neoplasia
|Funahashi H,Satake M,Dawson D,Huynh NA,Reber HA,Hines OJ,Eibl G||Cancer research (67:7068)||2007|
Inhibition of neuroblastoma cell proliferation with omega-3 fatty acids and treatment of a murine model of human neuroblastoma using a diet enriched with omega-3 fatty acids in combination with sunitinib.
MA5-14568 was used in western blot to study the anti-tumor activity of omega-3 fatty acids
|Barnés CM,Prox D,Christison-Lagay EA,Le HD,Short S,Cassiola F,Panigrahy D,Chaponis D,Butterfield C,Nehra D,Fallon EM,Kieran M,Folkman J,Puder M||Pediatric research (71:168)||2012|
Reversal of gene expression changes in the colorectal normal-adenoma pathway by NS398 selective COX2 inhibitor.
MA5-14568 was used in immunocytochemistry to investigate the modulating effect of NS398 on gene expression in HT29 cells
|Galamb O,Spisák S,Sipos F,Tóth K,Solymosi N,Wichmann B,Krenács T,Valcz G,Tulassay Z,Molnár B||British journal of cancer (102:765)||2010|