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Invitrogen
CRABP1 Monoclonal Antibody (C-1)
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FIGURE: 1 / 15
CRABP1 Antibody (MA3-813) in ICC/IF
Immunofluorescent analysis of CRABPI using Anti-CRABPI Monoclonal Antibody (C-1) (Product # MA3-813) shows staining in NIH-3T3 Cells. CRABPI staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CRABPI (Product # MA3-813) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA3-813
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Cat,
Human,
Mouse,
Non-human primate,
Rabbit,
Reptile,
Rat
Published species
Avian,
Human,
Monkey,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
C-1
Immunogen
Oxidized CRABPI from bovine retina.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography, Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-813 detects cellular retinoic acid binding protein I (CRABPI) from human, mouse, monkey, feline, bovine, rabbit, rat, turtle and chicken tissues. This antibody does not react with chameleon, goldfish and frog. This antibody does not cross-react with cellular retinol binding protein (CRBP), performic acid oxidized CRBP, interphotoreceptor retinoid binding protein or retinol binding protein.
MA3-813 has been successfully used in Western blot, immunohistochemistry and ELISA procedures. By Western blot, this antibody detects a 16 kDa protein representing CRABPI from rat retinal supernatant. Immunohistochemical staining of CRABPI in rat retina with MA3-813 results in its localization to amacrine somata and laminae in the inner plexiform layer.
The MA3-813 antigen is oxidized CRABPI from bovine retina.
Target Information
This gene encodes a specific binding protein for a vitamin A family member and is thought to play an important role in retinoic acid-mediated differentiation and proliferation processes. It is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid at specific sites within the nucleus, which may contribute to vitamin A-directed differentiation in epithelial tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cellular retinoic acid binding protein I (CRABP-I) homologue; cellular retinoic acid-binding protein I; CRABP-I; CRABP1; retinoic acid-binding protein; unnamed protein product
Gene Aliases: cCRABP-I; CRABP; Crabp-1; CRABP-I; CRABPI; Rbp-5; RBP5
UniProt ID: (Bovine) P62964, (Human) P29762, (Chicken) P40220, (Rat) P62966, (Mouse) P62965
Entrez Gene ID: (Bovine) 282201, (Human) 1381, (Rabbit) 100338607, (Chicken) 374211, (Rat) 25061, (Mouse) 12903
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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