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Western blot analysis of CREB in NIH-3T3 cells treated with PDGF (30ug) using a CREB recombinant rabbit monoclonal antibody (Product # 700129) at a dilution of 0.01ug/ml. NBT/BCIP was used as the substrate (Product # WB7105). Results show a band at ~43kDa. The second ~37 kDa band is seen in many cell types and is probably a close member of the ATF family. Competition assays show the disappearance of both the bands.
|Tested species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-3µg x 10^6 cells|
|Western Blot (WB)||1-3µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
CREB (cAMP responsive element binding protein) encodes for a 43 kDa protein transcription factor and belongs to leucine zipper family. CREB binds to the cyclic-AMP response element (CRE, a sequence present in many viral and cellular promoters) as a homodimer. Phosphorylated form of CREB act as an inducer of transcription for the genes, upon hormone dependent stimulation of cyclic-AMP pathway. CREB is known to play important role in circadian rythm generation and memory formation; aberration in CREB-mediated gene expression has been linked with Alzheimer disease. In humans, the gene is located on the q arm of chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Creb-1, Creb, 2310001E10Rik, 3526402H21Rik, CREB, AV083133
cAMP-responsive element-binding protein 1, cyclic AMP-responsive element-binding protein 1, CREB-1, active transcription factor CREB, cAMP-response element-binding protein-1, transactivator protein, Active transcription factor CREB, cAMP responsive element binding protein 1, CREB1, MGC9284, Transactivator protein