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CRP Antibody (MA5-42674) in IHC (P)
Immunohistochemistry analysis of CRP in paraffin-embedded human liver cancer. Samples were incubated with CRP Monoclonal antibody (Product # MA5-42674) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
MA5-42674
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
10J2R8
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 125-224 of human C-Reactive Protein (CRP) (P02741).
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911815
Product Specific Information
Positive test controls include: Human serum, Mouse serum. The target is usually found in the following locations: Secreted.
Immunogen sequence: VEFWVDGKPR VRKSLKKGYT VGAEASIILG QEQDSFGGNF EGSQSLVGDI GNVNMWDFVL SPDEINTIYL GGPFSPNVLN WRALKYEVQG EVFTKPQLWP
Target Information
CRP (C-reactive protein) is a major cyclic, pentameric acute phase protein compound consisting of five identical, noncovalently bound, nonglycosylated subunits. Eeach subunit is made up of 206 amino acids, and has a molecular weight of 24 kDa. CRP is produced by the liver, and its plasma levels rise dramatically during inflammatory processes occurring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.), plays an important role in innate immunity, and mediates activities associated with pre-immune nonspecific host resistance. Specifically, there is a 100-1000 fold increase of CRP in response to injury, infection or inflammation. CRP is opsonic, an initiator of the classical complement cascade, and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. CRP is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host, and initiates pathogen elimination by interacting with humoral and cellular effector systems in the blood. Patients with elevated basal levels of CRP have been shown to be an increased risk for hypertension and cardiovascular disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c reactive; C-reactive protein; C-reactive protein, petaxin related; CSRP; CYRP; D1S181E; DKFZp686M148; DKFZp686M149; MGC149895; MGC88244; pentraxin 1; RP11-419N10.4
Gene Aliases: AI255847; CRP; PTX1
UniProt ID: (Human) P02741, (Mouse) P14847
Entrez Gene ID: (Human) 1401, (Mouse) 12944
acute-phase response
complement activation, classical pathway
positive regulation of gene expression
negative regulation of macrophage derived foam cell differentiation
negative regulation of lipid storage
regulation of low-density lipoprotein particle clearance
negative regulation of superoxide anion generation
positive regulation of superoxide anion generation
wound healing
positive regulation of nitric oxide biosynthetic process
negative regulation of vasodilation
protein polymerization
cellular response to calcium ion
positive regulation of dendrite development
regulation of interleukin-8 secretion
inflammatory response
opsonization
defense response to Gram-positive bacterium
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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