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CRX Antibody (H00001406-M01) in WB
Western blot analysis of CRX over-expressed 293 cell line, cotransfected with CRX Validated Chimera RNAi (Cat # H00001406-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CRX monoclonal antibody (M01), clone F6-C2 (Cat # H00001406-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Product Details
H00001406-M01
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
F6-C2
Immunogen
CRX (AAH16664, 1 a.a. approximately 300 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: MMAYMNPGPH YSVNALALSG PSVDLMHQAV PYPSAPRKQR RERTTFTRSQ LEELEALFAK TQYPDVYARE EVALKINLPE SRVQVWFKNR RAKCRQQRQQ QKQQQQPPGG QAKARPAKRK AGTSPRPSTD VCPDPLGISD SYSPPLPGPS GSPTTAVATV SIWSPASESP LPEAQRAGLV ASGPSLTSAP YAMTYAPASA FCSSPSAYGS PSSYFSGLDP YLSPMVPQLG GPALSPLSGP SVGPSLAQSP TSLSGQSYGA YSPVDSLEFK DPTGTWKFTY NPMDPLDYKD QSAWKFQIL
Target Information
The cone-rod homeobox-containing gene (CRX) encodes a transcription factor that coordinates the expression of several photoreceptor genes in the developing retina, including opsin and rhodopsin. Specifically, CRX binds the OTX motif (TAATCC/A) upstream from photoreceptor genes. The CRX gene is also expressed in the pinealocytes of the pineal gland and may regulate pineal circadian activity by controling the expression of melatonin synthesis genes. Furthermore, CRX(-) mice exhibit disruption of circadian rhythms. The human CRX gene maps to chromosome 19q13.3 within the region of the cone-rod dystrophy-2 locus (CORD2). Mutations in the CRX gene are implicated in the visual pathologies of CORD, Leber congenital amaurosis (LCA) and retinitis pigmentosa (RP). All characterized CRX gene mutations produce disease in heterozygotes although there is no known correlation between the pathologic phenotype and genetic mutation. Missense mutations of the CRX gene affect the homeobox domain, whereas frameshift mutations affect the OTX domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cone rod homeobox; Cone-rod homeobox protein; CORD 2; LCA 7; orthodenticle homeobox 3; OTX; OTX 3
Gene Aliases: CORD2; CRD; CRX; LCA7; OTX3
UniProt ID: (Human) O43186
Entrez Gene ID: (Human) 1406
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
nuclear hormone receptor binding
leucine zipper domain binding
homeodomain transcription factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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