|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 364-392 amino acids from the C-terminal region of human CYP20A1|
|Purification||Ammonium sulfate precipitation, Size-exclusion - Dialysis|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
CYP20A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D,E)T, suggesting that its substrate may carry its own oxygen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Proteome-wide mapping of cholesterol-interacting proteins in mammalian cells.
PA5-14356 was used in western blot to use a chemoproteomic approach to map the cholesterol-interacting proteins present in mammalian cells
|Hulce JJ,Cognetta AB,Niphakis MJ,Tully SE,Cravatt BF||Nature methods (10:259)||2013|