Immunofluorescence analysis of Cadherin Pan was done on 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cadherin Pan Rabbit Polyclonal Antibody (PA516766) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Bovine, Dog, Chicken, Human, Mouse, Non-human primate, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the C-terminus of chicken N-cadherin|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16766 targets Cadherin Pan in IHC (P) and WB applications and shows reactivity with Bovine, Canine, Chicken, Human, mouse, Non-human primate, Rat, and Xenopus laevis samples. This antibody recognizes Cadherin E, N, P and R (Cadherin 1, 2, 3 and 4).
The PA5-16766 immunogen is a synthetic peptide from the C-terminus of chicken N-cadherin.
Cadherins are calcium dependent cell adhesion molecules, which play important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. In adhesion junctions, cadherins are bound to Beta and gamma catenins, which in turn bind to alpha catenin, an actin binding protein. Cadherins play important role in tumor invasion and metastasis. This antibody is excellent for staining of formalin-fixed, paraffin-embedded tissues.
IP-MS enrichment using a Cadherin pan antibody (LFQ intensity): CDH1 was enriched 190-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and Cadherin pan antibody (Part No. PA5-16766). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
IP-MS enrichment using a Cadherin pan antibody (LFQ intensity): CDH4, CDH1, and CDH2 were enriched 1162-fold, 288-fold, and 253-fold, respectively, from A549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and Cadherin pan antibody (Part No. PA5-16766). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
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