Western blotting by monoclonal antibodies specific to Calcitonin following 15% SDS-PAGE in reducing conditions. Antigen loaded - 100 ng/lane; Lane 1: Calcitonin antibody (Product # MA1-20285); Lane 2: Calcitonin antibody (Product # MA1-20890); Lane 3: Calcitonin antibody (Product # MA1-20891); Lane 4: Calcitonin antibody (Product # MA1-20290); Lane 5: Calcitonin antibody (Product # MA1-20288); Lane 6: Calcitonin antibody (Product # MA1-20291).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant, full length, human calcitonin protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20285 detects calcitonin in human samples.
MA1-20285 has been successfully used in ELISA and Western blot procedures. This antibody may be used in a sandwich ELISA when paired with MA1-20291 as the capture antibody.
The MA1-20285 immunogen is recombinant, full length, human calcitonin protein.
Calcitonin is a peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.