Immunofluorescent analysis of Calcium binding protein P22 showing staining in the cytoplasm of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Calcium binding protein P22 polyclonal antibody (Product # PA5-29876) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 195 of Human Calcium binding protein P22|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29876 targets Calcium binding protein P22 in IF, IHC (P), and WB applications and shows reactivity with Human, mouse and rat samples.
The PA5-29876 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 195 of Human Calcium binding protein P22.
This gene encodes a phosphoprotein that binds to the Na+/H+ exchanger NHE1. This protein serves as an essential cofactor which supports the physiological activity of NHE family members and may play a role in the mitogenic regulation of NHE1. The protein shares similarity with calcineurin B and calmodulin and it is also known to be an endogenous inhibitor of calcineurin activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.