|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A fusion protein containing the first 32 amino acid residues from rat CB2.|
|Storage buffer||PBS with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-746A detects cannabinoid receptor 2 (CB2) from human and rat tissues as well as transfected rat CB2.
PA1-746A has been successfully used in WB, immunocytochemistry, immunohistochemistry (paraffin) and FACS procedures. Immunocytochemical staining of CB2 into AtT20 cells transfected with the rat CB2 gene with PA1-746 yields a pattern consistent with plasma membrane staining.
PA1-746A antigen is a fusion protein containing the first 32 amino acid residues from rat CB2.
Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P/Q type calcium channels.
CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Islet cannabinoid receptors: cellular distribution and biological function.
PA1-746A was used in immunohistochemistry to study the expression of cannabinoid receptors in rat islets and their physiological significance
|Flores LE,Alzugaray ME,Cubilla MA,Raschia MA,Del Zotto HH,Román CL,Suburo AM,Gagliardino JJ||Pancreas (42:1085)||2013|
Overexpression of cannabinoid CB2 receptor in the brain induces hyperglycaemia and a lean phenotype in adult mice.
PA1-746A was used in immunohistochemistry to study the effects of CB2 receptor overexpression in murine brain on body mass and glucose metabolism
|Romero-Zerbo SY,Garcia-Gutierrez MS,Suárez J,Rivera P,Ruz-Maldonado I,Vida M,Rodriguez de Fonseca F,Manzanares J,Bermúdez-Silva FJ||Journal of neuroendocrinology (24:1106)||2012|
Involvement of the endocannabinoid system in periodontal healing.
PA1-746A was used in immunohistochemistry to study the role of endocannabinoid receptors in periodontal healing
|Kozono S,Matsuyama T,Biwasa KK,Kawahara K,Nakajima Y,Yoshimoto T,Yonamine Y,Kadomatsu H,Tancharoen S,Hashiguchi T,Noguchi K,Maruyama I||Biochemical and biophysical research communications (394:928)||2010|
Dual effect of anandamide on rat placenta nitric oxide synthesis.
PA1-746A was used in western blot to study the effect of anandamide on nitric oxide synthesis in rat placenta
|Cella M,Leguizamón GF,Sordelli MS,Cervini M,Guadagnoli T,Ribeiro ML,Franchi AM,Farina MG||Placenta (29:699)||2008|