Invitrogen

Cardiac Myosin Heavy Chain Monoclonal Antibody (3-48)

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Cardiac Myosin Heavy Chain Antibody in Western Blot (WB) — Figure 2 HQQR improves Ang II-induced cardiomyocyte hypertrophy. (A) Immunofluorescence experiments were performed using anti-alpha-actinin antibodies to analyze cardiomyocyte hypertrophy (magnification, x200; scale bar, 50 um). (B) mRNA expression levels of the myocardial hypertrophy markers ANP, BNP and beta-MHC were examined using reverse transcription-quantitative PCR. (C) Protein expression levels of the myocardial hypertrophy markers ANP, BNP and beta-MHC were examined using western blotting. ** P<0.01 and *** P<0.001 vs. the vehicle group; # P<0.05, ## P<0.01 and ### P<0.001 vs. the Ang II + vehicle group. HQQR, Huoxue Qianyang Qutan recipe; Ang II, angiotensin II; ANP, atrial natriuretic peptide; BNP, brain natriuretic peptide; beta-MHC, beta-major histocompatibility complex.

Product Details

MA1-26180

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Pig, Rabbit, Rat

Published species

Human, Mouse, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3-48

Immunogen

Purified, full length, native human protein.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2147173

Product Specific Information

This antibody is myosin alpha and beta heavy chain specific. Affinity constants: Human ventricular myosin heavy chains - 3.33 x 108M-1; Human atrial myosin heavy chains - 1.48 x 108M-1; Human skeletal muscle myosin - 1.06 x 108M-1; Canine cardiac myosin heavy chains - 2.06 x 108M-1. This antibody cross-reacts with heavy chains of human myosin isolated from slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. It does not react with human platelets, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction, nor does it react with any other human tissue antigens or blood cells.

Recommended positive control: Slow skeletal muscle, fast skeletal muscle, eye muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Huoxue Qianyang Qutan recipe attenuates Ang II-induced cardiomyocyte hypertrophy by regulating reactive oxygen species production.

Experimental and therapeutic medicine

Gui M,Yao L,Lu B,Wang J,Zhou X,Li J,Dong Z,Fu D

Published figure using Cardiac Myosin Heavy Chain monoclonal antibody (Product # MA1-26180) in Western Blot

Wed Dec 01 00:00:00 EST 2021

Intercellular cGAMP transmission induces innate immune activation and tissue inflammation in Trex1 deficiency.

iScience

Jütte BB,Krollmann C,Cieslak K,Koerber RM,Boor P,Graef CM,Bartok E,Wagner M,Carell T,Landsberg J,Aymans P,Wenzel J,Brossart P,Teichmann LL

MA1-26180 was used in ELISA to demonstrate that, in Trex1 -/- -associated autoimmunity, cGAMP shuttling from radioresistant to immune cells induces NF-κB activation, interferon regulatory factor 3 (IRF3) phosphorylation, and subsequent interferon signaling. cGAMP travel prevented myeloid cell and lymphocyte death, promoting their accumulation in secondary lymphoid tissue.

Fri Aug 20 00:00:00 EDT 2021

Cardiosphere-derived exosomal microRNAs for myocardial repair in pediatric dilated cardiomyopathy.

Science translational medicine

Hirai K,Ousaka D,Fukushima Y,Kondo M,Eitoku T,Shigemitsu Y,Hara M,Baba K,Iwasaki T,Kasahara S,Ohtsuki S,Oh H

MA1-26180 was used in Immunohistochemistry to determine the safety and efficacy of cardiosphere-derived cells in a porcine model of dilated cardiomyopathy and translate the preclinical results into this patient population.

Wed Dec 09 00:00:00 EST 2020

Cardiosphere-derived exosomal microRNAs for myocardial repair in pediatric dilated cardiomyopathy.

Science translational medicine

Hirai K,Ousaka D,Fukushima Y,Kondo M,Eitoku T,Shigemitsu Y,Hara M,Baba K,Iwasaki T,Kasahara S,Ohtsuki S,Oh H

Wed Dec 09 00:00:00 EST 2020

Defining the molecular signatures of human right heart failure.

Life sciences

Williams JL,Cavus O,Loccoh EC,Adelman S,Daugherty JC,Smith SA,Canan B,Janssen PML,Koenig S,Kline CF,Mohler PJ,Bradley EA

MA1-26180 was used in Western Blotting to provide some of the first analyses of the molecular heterogeneity between human RV and LV tissue, as well as key differences in human disease (RVF secondary to pulmonary hypertension and LVAD mediated RVF).

Thu Mar 01 00:00:00 EST 2018

Generation of multipotent induced cardiac progenitor cells from mouse fibroblasts and potency testing in ex vivo mouse embryos.

Nature protocols

Lalit PA,Rodriguez AM,Downs KM,Kamp TJ

MA126180 was used in immunocytochemistry to develop a protocol to generate expandable and multipotent induced cardiac progenitor cells from mouse adult fibroblasts

Mon May 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: alpha cardiac MHC; alpha myosin; alpha-cardiac myosin heavy chain; alpha-myosin; alpha-myosin heavy chain; alpha-myosin heavy chain subfragment 2 exon; Antigen MLAA-21; beta myosin heavy chain; beta-myosin; beta-myosin heavy chain; cardiac muscle myosin heavy chain 7 beta; cardiac myosin heavy chain alpha isoform; cardiac myosin heavy chain beta isoform; cardiomyopathy, hypertrophic 1; MYH-beta/slow; MyHC-alpha; MyHC-beta; MyHC-slow; myopathy, distal 1; Myosin cardiac muscle beta chain; Myosin heavy chain 6; Myosin heavy chain 7; Myosin heavy chain 7B, cardiac muscle beta isoform; myosin heavy chain polypeptide 6 cardiac muscle adult; myosin heavy chain polypeptide 7 cardiac muscle fetal; Myosin heavy chain slow isoform; Myosin heavy chain, cardiac muscle alpha isoform; Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha); Myosin heavy chain, cardiac muscle beta isoform; myosin heavy chain, cardiac muscle, adult; myosin heavy chain, cardiac muscle, fetal; myosin heavy chain, polypeptide 6, cardiac muscle, alpha; myosin heavy chain, polypeptide 7; myosin, heavy chain 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1); myosin, heavy chain 7B, cardiac muscle, beta; myosin, heavy polypeptide 6, cardiac muscle, alpha; myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1); myosin, heavy polypeptide 7, cardiac muscle, beta; myosin, heavy polypeptide 7B, cardiac muscle, beta; Myosin-6; Myosin-7; Myosin-7B; rhabdomyosarcoma antigen MU-RMS-40.7A; Slow A MYH14; slow type myosin heavy chain; type slow/beta myosin heavy chain; U937-associated antigen

Gene Aliases: A830009F23Rik; AA517445; alpha-MHC; ASD3; B-MHC; beta-MHC; CMD1EE; CMD1S; CMH1; CMH14; KIAA1512; MHC14; MPD1; MYH14; MYH6; MYH7; MYH7B; MYHC; Myhc-a; Myhc-b; MyHC-I; MYHCA; MYHCB; SPMD; SPMM; SSS3

UniProt ID: (Human) P13533, (Bovine) Q9BE39, (Human) P12883, (Pig) P79293, (Dog) P49824, (Human) A7E2Y1, (Mouse) Q02566, (Rat) P02564, (Mouse) Q91Z83, (Mouse) A2AQP0