|Tested species reactivity||Human|
|Published species reactivity||Rabbit, Rat, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant full length human Caspase 3 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:25-1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11516 targets Caspase 3 in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Human samples.
The MA5-11516 immunogen is recombinant full length human Caspase 3 protein.
Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kDa) proenzyme. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kDa and 12kDa, respectively. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The protective effects of carnosine in alcohol-induced hepatic injury in rats.
MA5-11516 was used in immunohistochemistry to study the mechanisms underlying the protective effects of carnosine in a rat model of alcohol-induced liver damage
|Baykara B,Micili SC,Tugyan K,Tekmen I,Bagriyanik H,Sonmez U,Sonmez A,Oktay G,Yener N,Ozbal S||Toxicology and industrial health (30:25)||2014|
Treatment with thyroxine restores myelination and clinical recovery after intraventricular hemorrhage.
MA5-11516 was used in immunohistochemistry and western blot to study the mechanisms by which thyroxine promotes myelination and neurological recovery in a rabbit model of intraventricular hemhorrage
|Vose LR,Vinukonda G,Jo S,Miry O,Diamond D,Korumilli R,Arshad A,Zia MT,Hu F,Kayton RJ,La Gamma EF,Bansal R,Bianco AC,Ballabh P||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:17232)||2013|
Erythropoietin prevents lymphoid apoptosis but has no effect on survival in experimental sepsis.
MA5-11516 was used in immunohistochemistry to study the lack of effect of EPO on survival in a rat model of sepsis despite its ability to inhibit lymphoid apoptosis
|Köro¿lu TF,Y¿lmaz O,Gökmen N,Tu¿yan K,Bask¿n H,E¿rilmez MY||Pediatric research (74:148)||2013|
Survival, integration, and differentiation of unrestricted somatic stem cells in the heart.
MA5-11516 was used in immunohistochemistry to study the ability of unrestricted somatic stem cells to survive and differentiate following trans-coronary injection in a rat model
|Ding Z,Burghoff S,Buchheiser A,Kögler G,Schrader J||Cell transplantation (22:15)||2013|
Captopril and telmisartan treatments attenuate cadmium-induced testicular toxicity in rats.
MA5-11516 was used in immunohistochemistry to study the mechanism by which captopril and telmisartan protect against testicular toxicity induced by cadmium exposure
|Fouad AA,Jresat I||Fundamental and clinical pharmacology (27:152)||2013|
Notch signaling change in pulmonary vascular remodeling in rats with pulmonary hypertension and its implication for therapeutic intervention.
MA5-11516 was used in immunohistochemistry to study the role of Notch signaling in pulmonary vascular remodeling in a rat hypoxia-induced model of pulmonary hypertension
|Qiao L,Xie L,Shi K,Zhou T,Hua Y,Liu H||PloS one (7:null)||2012|
Clinicopathological significance of cathepsin D expression in non-small cell lung cancer is conditional on apoptosis-associated protein phenotype: an immunohistochemistry study.
MA5-11516 was used in immunohistochemistry to study the influence of the phenotype of apoptosis-associated proteins on the clinical significance of cathepsin D expression in non-small cell lung cancer
|Fan C,Lin X,Wang E||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (33:1045)||2012|
Cadmium induces apoptosis in the pituitary gland of Podarcis sicula.
MA5-11516 was used in immunohistochemistry to investigate the toxic effect of Cadmium on the pituitary gland of Podarcis sicula
|Ferrandino I,Favorito R,Annunziata M,Grimaldi MC||Annals of the New York Academy of Sciences (1163:386)||2009|
Increased apoptosis in human osteoarthritic cartilage corresponds to reduced cell density and expression of caspase-3.
MA5-11516 was used in immunohistochemistry to investigate cell death in osteoarthritic cartilage and the role of caspase-3 during this process
|Sharif M,Whitehouse A,Sharman P,Perry M,Adams M||Arthritis and rheumatism (50:507)||2004|
Bioengineering functional copolymers. XXI. Synthesis of a novel end carboxyl-trithiocarbonate functionalized poly(maleic anhydride) and its interaction with cancer cells.
MA5-11516 was used in immunocytochemistry to study the synthesis of a novel bioengineering copolymer with anti-tumor activity and its interaction with cancer cells
|Rzayev ZM,T¿rk M,Söylemez EA||Bioorganic and medicinal chemistry (20:5053)||2012|
Bioengineering functional copolymers. XIV. Synthesis and interaction of poly(N-isopropylacrylamide-co-3,4-dihydro-2H-pyran-alt-maleic anhydride)s with SCLC cancer cells.
MA5-11516 was used in immunocytochemistry to synthesize novel supramolecularlly structured copolymers with anti-cancer activities and study their effects on human lung small cell carcinoma cells
|Türk M,Rzayev ZM,Khalilova SA||Bioorganic and medicinal chemistry (18:7975)||2010|
Baicalin induces apoptosis in leukemia HL-60/ADR cells via possible down-regulation of the PI3K/Akt signaling pathway.
MA5-11516 was used in western blot to study the role of the PI3 kinase-Akt signaling pathway in the mechanism by which baicalin induces apoptosis in drug-resistant human myeloid leukemia cells
|Zheng J,Hu JD,Chen YY,Chen BY,Huang Y,Zheng ZH,Liu TB||Asian Pacific journal of cancer prevention : APJCP (13:1119)||2012|