A549 cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ONTARGETplus CDK6 siRNA (Lane 3). Western blot data for CyclophilinB is included as a control for equal protein loading.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant cdk6 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13338 targets Cdk6 in IF, IHC (F), IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-13338 immunogen is purified recombinant cdk6 protein.
Cyclin-dependent kinase 6 (p40cdk6 also known as PLSTIRE) belongs to a family of proteins designated as cdks are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MiR-34a chemosensitizes bladder cancer cells to cisplatin treatment regardless of p53-Rb pathway status.
MA5-13338 was used in western blot to study the role of MiR-34a in sensitizing bladder cancer cells to cisplatin treatment
|Vinall RL,Ripoll AZ,Wang S,Pan CX,deVere White RW||International journal of cancer (130:2526)||2012|
Cyclin D2 and cyclin D3 play opposite roles in mouse skin carcinogenesis.
MA5-13338 was used in western blot to study the opposing roles of cyclin D2 and cyclin D3 in mouse skin carcinogenesis
|Rojas P,Cadenas MB,Lin PC,Benavides F,Conti CJ,Rodriguez-Puebla ML||Oncogene (26:1723)||2007|
|Not Applicable||Not Cited||
p16INK4a-induced senescence is disabled by melanoma-associated mutations.
MA5-13338 was used in immunocytochemistry and western blot to study the effect of melanoma-associated mutations on p16INK4a-induced senescence
|Haferkamp S,Becker TM,Scurr LL,Kefford RF,Rizos H||Aging cell (7:733)||2008|