Reconstituted by adding 0.5 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Cholesterol Oxidase is a bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product. This protein is involved in the pathway cholesterol metabolism, which is part of Steroid metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.