|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding human chromogranin A|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is pancreas tissue.
Chromogranin A (a protein of 439 amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: betagranin (N-terminal fragment of chromogranin A); catestatin; CgA; chromofungin; Chromogranin-A; parathyroid secretory protein 1; Pituitary secretory protein I; SP-I
Gene Aliases: CGA; CHGA
UniProt ID: (Human) P10645
Entrez Gene ID: (Human) 1113
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