|Tested species reactivity||Human|
|Published species reactivity||Dog|
|Host / Isotype||Mouse / IgM|
|Immunogen||Collagen type IV.|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent.|
|Immunohistochemistry (Frozen) (IHC (F))||1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
This antibody does not cross-react with Collagens I, II, III, or V.
For immunohistochemistry on frozen sections, fixation with acetone for 5 minutes at -20°C is recommended. Immunohistochemistry on paraffin sections has worked, but is less effective than staining on frozen tissues.
Reconstitute with 1 ml of distilled water. Store reconstituted product at 4°C.
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Usefulness of collagen IV immunostaining for diagnosis of canine epidermolysis bullosa acquisita.
MA1-35333 was used in immunohistochemistry to investigate the application of collagen IV immunostaining as a diagnostic marker for epidermolysis bullosa acquisita
|Olivry T,Dunston SM||Veterinary pathology (47:565)||2010|