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Invitrogen
Connexin 26 Monoclonal Antibody (CX-12H10)
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FIGURE: 1 / 47
Connexin 26 Antibody (13-8100) in WB
Western blot analysis of Connexin26 (Cx26) was performed by loading 30 µg of protein from mouse liver homogenate (not boiled) and 7 µL of EZ-RUN Prestained Rec Protein Ladder per well onto a 10% Tris-Hcl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked in blocking buffer composed of 20 mM Tris-HCl pH 8, 150 mM NaCl and 0.2% Tween-20 (TBST) supplemented with 5% skim milk powder (Carnation) for 1 hr at room temperature. Cx26 was detected at ~26 kDa using an anti-Cx26 antibody (Product # 13-8100, lane 1) ... View More
Product Details
13-8100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bat,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
CX-12H10
Immunogen
Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
13-8100 reacts strongly with Connexin-26, which has a predicted molecular weight of 26.5 kDa. Reactivity of this antibody on western blots has been confirmed by using extracts from mouse liver, mouse brain, and rat brain and it appears to exhibit minimal cross-reaction with Cx30 by western blotting. The degree of cross-reaction with Cx30 by IHC is uncertain, but may be influenced by fixation conditions.
Target Information
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Defects in GJB3 have been linked to erythrokeratodermia variables (EKV) is an autosomal dominant genodermatosis characterized by transient figurate red patches or hyperkeratosis. Mutations in GJB2 have also been associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: connexin 26; Connexin-26; Cx26; Gap junction beta-2 protein; gap junction channel protein connexin 26; gap junction membrane channel protein beta 2; gap junction protein, beta 2, 26kDa; mutant gap junction protein beta 2
Gene Aliases: AI325222; Cnx26; CX26; CXN-26; DFNA3; DFNA3A; DFNB1; DFNB1A; Gjb-2; GJB2; HID; KID; NSRD1; PPK
UniProt ID: (Human) P29033, (Mouse) Q00977, (Rat) P21994
Entrez Gene ID: (Human) 2706, (Mouse) 14619, (Rat) 394266
transport
cell-cell signaling
sensory perception of sound
gap junction assembly
male genitalia development
response to estradiol
response to progesterone
cellular response to oxidative stress
response to human chorionic gonadotropin
decidualization
transmembrane transport
cell communication
female pregnancy
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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