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Immunofluorescence analysis of Connexin 26/GJB2 was done on 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Connexin 26/GJB2 Rabbit polyclonal Antibody (512800) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing junctional localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human , Mouse , Rat|
|Published species reactivity||Rat , Mouse , Clawed frog|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 13 amino acid synthetic peptide derived from the C-terminus of the mouse Connexin 26 protein. This mouse sequence differs from the rat sequence by a single amino acid and from the human sequence by two (non-consecutive) amino acids.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||10-20 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 4 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Western Blot (WB)||See 4 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 1 publications below|
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Defects in GJB3 have been linked to erythrokeratodermia variabilis (EKV) is an autosomal dominant genodermatosis characterized by transient figurate red patches or hyperkeratosis. Mutations in GJB2 have also been associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Timed conditional null of connexin26 in mice reveals temporary requirements of connexin26 in key cochlear developmental events before the onset of hearing.
51-2800 was used in immunocytochemistry to show that Gjb2 is required for normal Corti development in mice
|Chang Q,Tang W,Kim Y,Lin X||Neurobiology of disease (73:418)||2015|
Mammary gland specific knockdown of the physiological surge in Cx26 during lactation retains normal mammary gland development and function.
51-2800 was used in immunocytochemistry to determine if patients with loss-of-function Cx26 mutations have a greater susceptibility to impaired breast development
|Stewart MK,Plante I,Bechberger JF,Naus CC,Laird DW||PloS one (9:null)||2014|
Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.
51-2800 was used in immunocytochemistry to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus
|Bautista W,Rash JE,Vanderpool KG,Yasumura T,Nagy JI||The European journal of neuroscience (39:757)||2014|
Connexin43 reduces melanoma growth within a keratinocyte microenvironment and during tumorigenesis in vivo.
51-2800 was used in immunocytochemistry to study the role of connexins in melanoma tumorigenesis and assess their status during cancer onset and progression.
|Ableser MJ,Penuela S,Lee J,Shao Q,Laird DW||The Journal of biological chemistry (289:1592)||2014|
Connexin 26 null mice exhibit spiral ganglion degeneration that can be blocked by BDNF gene therapy.
51-2800 was used in immunohistochemistry to characterize mice that lack connexin 26 in the non-sensory cells flanking the auditory epithelium.
|Takada Y,Beyer LA,Swiderski DL,O'Neal AL,Prieskorn DM,Shivatzki S,Avraham KB,Raphael Y||Hearing research (309:124)||2014|
BAAV mediated GJB2 gene transfer restores gap junction coupling in cochlear organotypic cultures from deaf Cx26Sox10Cre mice.
51-2800 was used in immunohistochemistry to study the expression and function of connexin26 and connexin30 in the ears of transgenic mice.
|Crispino G,Di Pasquale G,Scimemi P,Rodriguez L,Galindo Ramirez F,De Siati RD,Santarelli RM,Arslan E,Bortolozzi M,Chiorini JA,Mammano F||PloS one (6:null)||2011|
Localization of connexin26 and connexin32 in putative CO(2)-chemosensitive brainstem regions in rat.
||Solomon IC,Halat TJ,El-Maghrabi MR,O'Neal MH||Respiration physiology (129:101)||2001|
Cerebral ischemic injury is enhanced in a model of oculodentodigital dysplasia.
51-2800 was used in western blot to assess the effect of the +/G60S mutation in connexin 43 following stroke.
|Kozoriz MG,Lai S,Vega JL,Sáez JC,Sin WC,Bechberger JF,Naus CC||Neuropharmacology (75:549)||2013|
|Clawed frog||Not Cited||
The D50N mutation and syndromic deafness: altered Cx26 hemichannel properties caused by effects on the pore and intersubunit interactions.
||Sanchez HA,Villone K,Srinivas M,Verselis VK||The Journal of general physiology (142:3)||2013|
Connexin26 expression in brain parenchymal cells demonstrated by targeted connexin ablation in transgenic mice.
51-2800 was used in western blot to study connexin 26 in astrocytes.
|Nagy JI,Lynn BD,Tress O,Willecke K,Rash JE||The European journal of neuroscience (34:263)||2011|
Expression of connexins in embryonic mouse neocortical development.
51-2800 was used in western blot to study of the spatial and temporal pattern of connexin expression in the developing brain.
|Cina C,Bechberger JF,Ozog MA,Naus CC||The Journal of comparative neurology (504:298)||2007|
Focal adhesion kinase modulates radial glia-dependent neuronal migration through connexin-26.
51-2800 was used in immunohistochemistry - frozen section to examine the function of focal adhesion kinase in cortical interneurons and pyramidal cells.
|Valiente M,Ciceri G,Rico B,Marín O||The Journal of neuroscience : the official journal of the Society for Neuroscience (31:11678)||2011|
Exocrine specific expression of Connexin32 is dependent on the basic helix-loop-helix transcription factor Mist1.
||Rukstalis JM,Kowalik A,Zhu L,Lidington D,Pin CL,Konieczny SF||Journal of cell science (116:3315)||2003|
NSRD1, CX26, Cnx26, HID, DFNB1A, PPK, Gjb-2, CXN-26, DFNA3A, KID, DFNB1, DFNA3, AI325222, Cx26
connexin 26, gap junction beta-2 protein, gap junction protein beta 2, connexin-26, gap junction channel protein connexin 26, gap junction membrane channel protein beta 2, GJB2, Connexin 26, gap junction protein, beta 2, 26kDa (connexin 26), CX26, DFNA3, DFNB1, HID, KID, NSRD1, PPK, 26kD (connexin 26)