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Immunofluorescence was performed on sections of adult mouse retina. Tissue sections were probed overnight at 4°C with mouse monoclonal anti-Cx36 antibody (Cat. No.37-4600, 2 µg/ml), and detected with donkey anti-mouse Alexa Fluor® 488 conjugate. Images were taken on a Zeiss Z2 scanning microscope at 40X magnification, and demonstrate antibody labeling for Cx36 localized at gap junctions between neurons in the inner plexiform layer of mouse retina. Image courtesy of Dr. James Nagy, University of Manitoba, Winnipeg, Canada.
|Tested species reactivity||Mouse , Human|
|Published species reactivity||Rat , Sheep , Fish , Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide derived from the C-terminal region of the rat and mouse Connexin36 proteins.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2-3 µg/mL|
|Immunofluorescence (IF)||2-3 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
GJA9, also called connexin-36 (CX36), is a member of the connexin gene family that is expressed predominantly in mammalian neurons. Connexins associate in groups of 6 and are organized radially around a central pore to form connexons. Each gap junction intercellular channel is formed by the conjunction of 2 connexons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Maternal treatment with glucocorticoids modulates gap junction protein expression in the ovine fetal brain.
37-4600 was used in western blot to study the effect of maternal glucocorticoid treatment on gap junction protein expression in the ovine fetal brain
|Sadowska GB,Stonestreet BS||Neuroscience (275:248)||2014|
Diurnal and circadian regulation of connexin 36 transcript and protein in the mammalian retina.
37-4600 was used in western blot to test if retinal connexin 36 is regulated at the level of transcript and protein expression.
|Katti C,Butler R,Sekaran S||Investigative ophthalmology & visual science (54:821)||2013|
Connexin and AMPA receptor expression changes over time in the rat olfactory bulb.
37-4600 was used in western blot to test if circadian electrical oscillations are synchronized by changes in the expression of connexins and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunits.
|Corthell JT,Fadool DA,Trombley PQ||Neuroscience (222:38)||2012|
Abundance of gap junctions at glutamatergic mixed synapses in adult Mosquitofish spinal cord neurons.
37-4600 was used in immunohistochemistry to investigate the role of gap junctions in fast motor behavior
|Serrano-Velez JL,Rodriguez-Alvarado M,Torres-Vazquez II,Fraser SE,Yasumura T,Vanderpool KG,Rash JE,Rosa-Molinar E||Frontiers in neural circuits (8:null)||2014|
Subcellular distribution of connexin45 in OFF bipolar cells of the mouse retina.
37-4600 was used in immunohistochemistry to investigate the localization and function of Cx45 in OFF bipolar cells.
|Hilgen G,von Maltzahn J,Willecke K,Weiler R,Dedek K||The Journal of comparative neurology (519:433)||2011|
Neuronal connexin expression in the cochlear nucleus of big brown bats.
||Horowitz SS,Stamper SA,Simmons JA||Brain research (1197:76)||2008|
Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.
37-4600 was used in immunocytochemistry to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus
|Bautista W,Rash JE,Vanderpool KG,Yasumura T,Nagy JI||The European journal of neuroscience (39:757)||2014|
Connexins and steroidogenesis in mouse Leydig cells.
37-4600 was used in immunohistochemistry - paraffin section to identify connexins in mouse Leydig cells that control steroidogenesis.
|Li D,Sekhon P,Barr KJ,Márquez-Rosado L,Lampe PD,Kidder GM||Canadian journal of physiology and pharmacology (91:157)||2013|
Ultrastructural localization of connexins (Cx36, Cx43, Cx45), glutamate receptors and aquaporin-4 in rodent olfactory mucosa, olfactory nerve and olfactory bulb.
||Rash JE,Davidson KG,Kamasawa N,Yasumura T,Kamasawa M,Zhang C,Michaels R,Restrepo D,Ottersen OP,Olson CO,Nagy JI||Journal of neurocytology (34:307)||2005|
Gja9, GJA9, cx36, connexin36, CX36
connexin 36, connexin-36, gap junction alpha-9 protein, gap junction delta-2 protein, gap junction membrane channel protein alpha 9, gap junction protein, alpha 9, GJA10