Invitrogen

Cyclin H Recombinant Rabbit Monoclonal Antibody (SN20-48)

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Cyclin H Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of Cyclin H in PC-3M cells using a Cyclin H Monoclonal antibody (Product # MA5-32331) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Cyclin H Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Cyclin H of paraffin-embedded Human colon cancer tissue using a Cyclin-H Monoclonal antibody (Product #MA5-32331). Counter stained with hematoxylin.

Cyclin H Antibody in Western Blot (WB) — Western blot analysis of Cyclin H in K562 cell lysate using a Cyclin H Monoclonal antibody (Product # MA5-32331) at a dilution of 1:1,000.

Cyclin H Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of Cyclin H in Hela cells using a Cyclin H Monoclonal Antibody (Product # MA5-32331) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Cyclin H Antibody in Immunohistochemistry (IHC) — Fig. 1 Immunohistochemistry staining of Cyclin H in ovarian cancer tissues. The expression of Cyclin H in ovarian cancer of grade 1 ( a ), grade 2 ( b ), and grade 3 ( c ) was measured by immunohistochemistry staining, and representative images are displayed. Scale bar, 20 um. d Quantification of Cyclin H expression in ovarian cancer (P < 0.001)

Product Details

MA5-32331

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:5,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	1:50-1:200	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:100-1:500	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Expression System	HEK293 cells
Class	Recombinant Monoclonal
Type	Antibody
Clone	SN20-48
Immunogen	Recombinant protein within Human Cyclin H aa 230-323 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2809612

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cyclin H predicts the poor prognosis and promotes the proliferation of ovarian cancer.

Cancer cell international

Peng C,Yang Y,Ji L,Yang P,Yang X,Zhang Y

MA5-32331 was used in Western Blot, Immunohistochemistry to suggest that high expression of cyclin H predicts the poor prognosis and promotes the growth of ovarian cancer by regulating the cell cycle.

Fri Apr 15 00:00:00 EDT 2022

Cyclin H predicts the poor prognosis and promotes the proliferation of ovarian cancer.

Cancer cell international

Peng C,Yang Y,Ji L,Yang P,Yang X,Zhang Y

Fri Apr 15 00:00:00 EDT 2022

Cyclin H predicts the poor prognosis and promotes the proliferation of ovarian cancer.

Cancer cell international

Peng C,Yang Y,Ji L,Yang P,Yang X,Zhang Y

Fri Apr 15 00:00:00 EDT 2022

Cyclin H predicts the poor prognosis and promotes the proliferation of ovarian cancer.

Cancer cell international

Peng C,Yang Y,Ji L,Yang P,Yang X,Zhang Y

Fri Apr 15 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: CAK complex subunit; CDK-activating kinase complex subunit; cyclin-dependent kinase-activating kinase complex subunit; Cyclin-H; MO15-associated protein; OTTHUMP00000222395; OTTHUMP00000222396; p34; p37

Gene Aliases: 6330408H09Rik; AI661354; AV102684; AW538719; CAK; CCNH; CycH; p34; p37

UniProt ID: (Human) P51946, (Mouse) Q61458

Entrez Gene ID: (Human) 902, (Mouse) 66671

Molecular Function: kinase activator

Function(s)

G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex assembly transcription initiation from RNA polymerase I promoter transcription elongation from RNA polymerase I promoter termination of RNA polymerase I transcription transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping protein phosphorylation positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of transcription from RNA polymerase II promoter positive regulation of phosphorylation of RNA polymerase II C-terminal domain regulation of cyclin-dependent protein serine/threonine kinase activity transcription, DNA-templated regulation of transcription, DNA-templated cell cycle phosphorylation regulation of cell cycle

Process(es)

protein binding DNA-dependent ATPase activity RNA polymerase II carboxy-terminal domain kinase activity cyclin-dependent protein serine/threonine kinase regulator activity cyclin-dependent protein serine/threonine kinase activity kinase activity protein kinase binding

Cyclin H Recombinant Rabbit Monoclonal Antibody (SN20-48)

Product Details

MA5-32331

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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