|Tested species reactivity||Dog, Hamster, Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues S(42) G N P L V Y L D V D A N(54) C of mature human CyPD.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 11 publications below|
PA1-028 detects cyclophilin D (CyPD) in rat, hamster, canine, mouse, and human tissues.
PA1-028 has been sucessfully used in Western blot procedures. By Western blot, this antibody detects a ~15 kDa protein in rat liver and heart extract, and a ~37 kDa in rat brain extract.
The PA1-028 immunogen is a synthetic peptide corresponding to residues S(42) G N P L V Y L D V D A N(54) C of mature human CyPD. This peptide (Cat. # PEP-104) is available for use in neutralization and control experiments.
Cyclophilin D (CyPD) is a member of the immunophilin family of proteins. Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins which bind FK506 are termed FK506 Binding Proteins (FKBP's) and those which bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyPD is located in the inter-membrane space of the mitochondria where it has been shown to be an integral member of the permeability transition pore complex which also includes the voltage dependent anion channel (VDAC) and the adenine nucleotide translocator (ANT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mitochondrial permeability transition and its regulatory components are implicated in apoptosis of primary cultures of rat proximal tubular cells exposed to lead.
PA1-028 was used in western blot to expand our understanding of the mechanisms involved in Pb-induced mitochondrial apoptosis
|Liu G,Wang ZK,Wang ZY,Yang DB,Liu ZP,Wang L||Archives of toxicology (90:1193)||2016|
|Not Applicable||Not Cited||
Cardiac and mitochondrial dysfunction following acute pulmonary exposure to mountaintop removal mining particulate matter.
PA1-028 was used in western blot to analyze acute pulmonary exposure to mountaintop removal mining particulate matter and cardiac and mitochondrial dysfunction
|Nichols CE,Shepherd DL,Knuckles TL,Thapa D,Stricker JC,Stapleton PA,Minarchick VC,Erdely A,Zeidler-Erdely PC,Alway SE,Nurkiewicz TR,Hollander JM||American journal of physiology. Heart and circulatory physiology (309:H2017)||2015|
Cyclophilin D-sensitive mitochondrial permeability transition in adult human brain and liver mitochondria.
PA1-028 was used in western blot to investigate the effect of CypD on mitochondrial permeability regulation in liver and brain
|Hansson MJ,Morota S,Chen L,Matsuyama N,Suzuki Y,Nakajima S,Tanoue T,Omi A,Shibasaki F,Shimazu M,Ikeda Y,Uchino H,Elmér E||Journal of neurotrauma (28:143)||2011|
Mitochondrial dysfunction in the type 2 diabetic heart is associated with alterations in spatially distinct mitochondrial proteomes.
PA1-028 was used in western blot to investigate the association between spatially distinct mitochondrial proteome and its dysfunction in the type 2 diabetic heart
|Dabkowski ER,Baseler WA,Williamson CL,Powell M,Razunguzwa TT,Frisbee JC,Hollander JM||American journal of physiology. Heart and circulatory physiology (299:H529)||2010|
Enhanced apoptotic propensity in diabetic cardiac mitochondria: influence of subcellular spatial location.
PA1-028 was used in western blot to study the mitochondrial apoptotic status of myocardium in diabetes mellitus
|Williamson CL,Dabkowski ER,Baseler WA,Croston TL,Alway SE,Hollander JM||American journal of physiology. Heart and circulatory physiology (298:H633)||2010|
Developmental shift of cyclophilin D contribution to hypoxic-ischemic brain injury.
PA1-028 was used in western blot to study the different roles of cyclophilin D in hypoxic-ischemic brain injury between immature and adult brains
|Wang X,Carlsson Y,Basso E,Zhu C,Rousset CI,Rasola A,Johansson BR,Blomgren K,Mallard C,Bernardi P,Forte MA,Hagberg H||The Journal of neuroscience : the official journal of the Society for Neuroscience (29:2588)||2009|
Mitochondria-specific transgenic overexpression of phospholipid hydroperoxide glutathione peroxidase (GPx4) attenuates ischemia/reperfusion-associated cardiac dysfunction.
PA1-028 was used in western blot to investigate the effect of phospholipid hydroperoxide glutathione peroxidase on ischemia/reperfusion-associated cardiac dysfunction
|Dabkowski ER,Williamson CL,Hollander JM||Free radical biology and medicine (45:855)||2008|
Muscle denervation promotes opening of the permeability transition pore and increases the expression of cyclophilin D.
PA1-028 was used in western blot to examine the effects of muscle denervation on the sensitivity of the PTP to calcium-induced opening and the expression of cyclophilin D
|Csukly K,Ascah A,Matas J,Gardiner PF,Fontaine E,Burelle Y||The Journal of physiology (574:319)||2006|
Effects of permeability transition inhibition and decrease in cytochrome c content on doxorubicin toxicity in K562 cells.
PA1-028 was used in western blot to investigate the effects of permeability transition inhibition and decrease in cytochrome c content on doxorubicin toxicity in K562 cells
|De Oliveira F,Chauvin C,Ronot X,Mousseau M,Leverve X,Fontaine E||Oncogene (25:2646)||2006|
Properties of the permeability transition pore in mitochondria devoid of Cyclophilin D.
PA1-028 was used in western blot to study the permeability transition pore (PTP) and its modulation in vitro and in vivo.
|Basso E,Fante L,Fowlkes J,Petronilli V,Forte MA,Bernardi P||The Journal of biological chemistry (280:18558)||2005|
Bax does not directly participate in the Ca(2+)-induced permeability transition of isolated mitochondria.
PA1-028 was used in western blot to study the role of Bax in the calcium-induced mitochondrial permeability transition.
|De Marchi U,Campello S,Szabò I,Tombola F,Martinou JC,Zoratti M||The Journal of biological chemistry (279:37415)||2004|