This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details
45-6100 was used successfully in the western blot analysis of cytochrome C using human breast cancer cell line lysates.
Positive control: Isolated mitochondria from Human, Bovine, Rat and Mouse heart; Human fibroblasts; HeLa cells.
WB: Predicted molecular weight: 14 kDa. ICC/IF: Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cytochrome c; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; hc1
Gene Aliases: CYC; CYCS; CYCSA; cytc; HCS; THC4