Western blot of Cytohesin 2 in rat brain extract using a Cytohesin 2 monoclonal antibody (Product # MA1-25510) at a dilution of 5µg/ml and developed with Biotin APA mouse Fab ad/HuBov second antibody, an Alkaline Phosphatase conjugate as a tertiary reagent, and a NBT/BCIP substrate.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant, full length human protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Western Blot (WB)||5-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25510 detects Cytohesin 2 from human and rat samples.
MA1-25510 has been successfully used in immunocytochemistry, and Western blot procedures.
The MA1-25510 immunogen is recombinant, full length human protein.
The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with ARF1, ARF3, and ARF6 and is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.