|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A synthetic peptide from the C-terminus of human cytokeratin 16|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13730 targets Cytokeratin 16 in immunofluorescence and immunohistochemistry (paraffin) applications and shows reactivity with Human samples. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
The MA5-13730 immunogen is a synthetic peptide from the C-terminus of human cytokeratin 16.
Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Development of full-thickness human skin equivalents with blood and lymph-like capillary networks by cell coating technology.
MA5-13730 was used in immunohistochemistry to develop a 3D human skin equivalent containing blood and lymph-like capillary networks
|Matsusaki M,Fujimoto K,Shirakata Y,Hirakawa S,Hashimoto K,Akashi M||Journal of biomedical materials research. Part A (103:3386)||2015|
Interactions between myofibroblast differentiation and epidermogenesis in constructing human living skin equivalents.
MA5-13730 was used in immunohistochemistry to use a human living skin equivalents model to study myofibroblast differentiation and epidermogenesis
|Yang L,Hashimoto K,Tohyama M,Okazaki H,Dai X,Hanakawa Y,Sayama K,Shirakata Y||Journal of dermatological science (65:50)||2012|
Development of skin-humanized mouse models of pachyonychia congenita.
MA5-13730 was used in immunohistochemistry to develop and evaluate a mouse model for pachyonychia congenita
|García M,Larcher F,Hickerson RP,Baselga E,Leachman SA,Kaspar RL,Del Rio M||The Journal of investigative dermatology (131:1053)||2011|
Down-regulation of keratin 4 and keratin 13 expression in oral squamous cell carcinoma and epithelial dysplasia: a clue for histopathogenesis.
MA5-13730 was used in immunohistochemistry to study the expression of keratin 4 and keratin 13 in oral squamous cell carcinoma and epithelial dysplasia
|Sakamoto K,Aragaki T,Morita K,Kawachi H,Kayamori K,Nakanishi S,Omura K,Miki Y,Okada N,Katsube K,Takizawa T,Yamaguchi A||Histopathology (58:531)||2011|
Methods to promote Notch signaling at the biomaterial interface and evaluation in a rafted organ culture model.
MA5-13730 was used in immunohistochemistry to evaluate biomaterials suitable for promoting and altering Notch signaling pathway
|Beckstead BL,Tung JC,Liang KJ,Tavakkol Z,Usui ML,Olerud JE,Giachelli CM||Journal of biomedical materials research. Part A (91:436)||2009|
Proteomic analysis of scleroderma lesional skin reveals activated wound healing phenotype of epidermal cell layer.
MA5-13730 was used in immunohistochemistry to study proteomic changes in the skin of patients with sclerodermal skin lesions
|Aden N,Shiwen X,Aden D,Black C,Nuttall A,Denton CP,Leask A,Abraham D,Stratton R||Rheumatology (Oxford, England) (47:1754)||2008|
Keratinocyte migration, proliferation, and differentiation in chronic ulcers from patients with diabetes and normal wounds.
MA5-13730 was used in immunohistochemistry to investigate the characteristics of keratinocytes in chronic ulcers of diabetic and normally wounded patients
|Usui ML,Mansbridge JN,Carter WG,Fujita M,Olerud JE||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (56:687)||2008|
Morphological evidence for the role of suprabasal keratinocytes in wound reepithelialization.
MA5-13730 was used in immunohistochemistry to investigate the involvment of suprabasal keratinocytes in wound healing and closure
|Usui ML,Underwood RA,Mansbridge JN,Muffley LA,Carter WG,Olerud JE||Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society (13:468)||2005|
Mek1 alters epidermal growth and differentiation.
MA5-13730 was used in immunohistochemistry to investigate the influence of Mek1 on epidermal growth and differentiation
|Scholl FA,Dumesic PA,Khavari PA||Cancer research (64:6035)||2004|
A study of cytokeratin profiles in localized cutaneous amyloids.
MA5-13730 was used in immunohistochemistry to investigate the expression profile of cytokeratins in cutaneous amyloids
|Chang YT,Liu HN,Wang WJ,Lee DD,Tsai SF||Archives of dermatological research (296:83)||2004|
Organotypic keratinocyte coculture using normal human serum: an immunomorphological study at light and electron microscopic levels.
MA5-13730 was used in immunohistochemistry to develop an organotypic keratinocyte coculture system with potential for use in autologous skin grafting
|Hinterhuber G,Marquardt Y,Diem E,Rappersberger K,Wolff K,Foedinger D||Experimental dermatology (11:413)||2002|
Effects of antikeratin 16 antibodies on the expression of Toll-like receptors 2 and 4 in keratinocytes.
MA5-13730 was used in blocking or activating experiment to study the effect of antikeratin 16 antibodies on Toll-like receptors 2 and 4 in keratinocytes
|Wu C,Luan Q,Li C,Zheng Z||Clinical and experimental dermatology (34:236)||2009|
Innate immune modulation of keratinocytes by antikeratin 16 antibodies.
MA5-13730 was used in blocking or activating experiment to study the innate immune modulation of keratinocytes by antikeratin 16 antibodies and the significance in psoriasis
|Wu C,Li C,Wei L,Zheng Z||Experimental dermatology (17:645)||2008|