|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Human, Mouse, Not Applicable, Rhesus monkey|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human PMC-42 breat carcinoma Cells.|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 13 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Immunohistochemistry (IHC)||See 11 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Flow Cytometry (Flow)||See 2 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|ELISA (ELISA)||See 1 publications below|
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Vitamin D and androgen receptor-targeted therapy for triple-negative breast cancer.
MA1-12589 was used in western blot to characterize androgen receptor-targeted and vitamin D therapy for triple-negative breast cancer
|Thakkar A,Wang B,Picon-Ruiz M,Buchwald P,Ince TA||Breast cancer research and treatment (157:77)||2016|
Antagonistic roles of Notch and p63 in controlling mammary epithelial cell fates.
MA1-12589 was used in western blot to investigate the role of Notch and p63 in regulation of mammary epithelial cell fates
|Yalcin-Ozuysal O,Fiche M,Guitierrez M,Wagner KU,Raffoul W,Brisken C||Cell death and differentiation (17:1600)||2010|
Cytoskeletal keratin glycosylation protects epithelial tissue from injury.
MA1-12589 was used in western blot to study the role of cytoskeletal keratin glycosylation in protecting epithelial tissue from injury
|Ku NO,Toivola DM,Strnad P,Omary MB||Nature cell biology (12:876)||2010|
Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.
MA1-12589 was used in western blot to study the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines
|Chang CY,Chiou PP,Chen WJ,Li YH,Yiu JC,Cheng YH,Chen SD,Lin CT,Lai YS||Research in veterinary science (88:285)||2010|
Frzb, a secreted Wnt antagonist, decreases growth and invasiveness of fibrosarcoma cells associated with inhibition of Met signaling.
MA1-12589 was used in western blot to study the effect of the secreted Wnt antagonist Frzb on fibrosarcoma growth and metastasis
|Guo Y,Xie J,Rubin E,Tang YX,Lin F,Zi X,Hoang BH||Cancer research (68:3350)||2008|
Analysis of keratin polypeptides 8 and 19 variants in inflammatory bowel disease.
MA1-12589 was used in western blot to study whether variants of keratin 8 and keratin 19 are associated with inflammatory bowel disease
|Tao GZ,Strnad P,Zhou Q,Kamal A,Zhang L,Madani ND,Kugathasan S,Brant SR,Cho JH,Omary MB,Duerr RH||Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association (5:857)||2007|
Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture.
MA1-12589 was used in western blot to examine the biological responses imparted by Matrigel overlays on primary human hepatocyte cultures
|Page JL,Johnson MC,Olsavsky KM,Strom SC,Zarbl H,Omiecinski CJ||Toxicological sciences : an official journal of the Society of Toxicology (97:384)||2007|
Protein phosphatase-2A associates with and dephosphorylates keratin 8 after hyposmotic stress in a site- and cell-specific manner.
MA1-12589 was used in western blot to study the site-specific dephosphorylation of keratin 8 by protein phosphatase-2A and how this varies according to cell type
|Tao GZ,Toivola DM,Zhou Q,Strnad P,Xu B,Michie SA,Omary MB||Journal of cell science (119:1425)||2006|
Identification of 14-3-3sigma as a contributor to drug resistance in human breast cancer cells using functional proteomic analysis.
MA1-12589 was used in western blot to study the role of 14-3-3sigma in drug resistance in human breast cancer cells
|Liu Y,Liu H,Han B,Zhang JT||Cancer research (66:3248)||2006|
Expression of Frzb/secreted Frizzled-related protein 3, a secreted Wnt antagonist, in human androgen-independent prostate cancer PC-3 cells suppresses tumor growth and cellular invasiveness.
MA1-12589 was used in western blot to investigate the suppressive effects of Frzb/sFRP3 expression on tumor growth and cellular invasiveness in PC-3 cells
|Zi X,Guo Y,Simoneau AR,Hope C,Xie J,Holcombe RF,Hoang BH||Cancer research (65:9762)||2005|
Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease.
MA1-12589 was used in western blot to study keratin 8 and keratin 18 hyperphosphorylation as markers for progression of human liver disease
|Toivola DM,Ku NO,Resurreccion EZ,Nelson DR,Wright TL,Omary MB||Hepatology (Baltimore, Md.) (40:459)||2004|
Keratin mutation in transgenic mice predisposes to Fas but not TNF-induced apoptosis and massive liver injury.
MA1-12589 was used in western blot to study the role of keratin mutations in the predisposition to Fas-induced apoptosis and massive liver injury
|Ku NO,Soetikno RM,Omary MB||Hepatology (Baltimore, Md.) (37:1006)||2003|
Keratin 8 mutations in patients with cryptogenic liver disease.
MA1-12589 was used in western blot to study mutations of keratin 8 in cryptogenic liver disease patients
|Ku NO,Gish R,Wright TL,Omary MB||The New England journal of medicine (344:1580)||2001|
|Not Applicable||Not Cited||
Modelling breast cancer requires identification and correction of a critical cell lineage-dependent transduction bias.
MA1-12589 was used in immunocytochemistry to study and identify critical cell lineage-dependent transduction bias to model breast cancer properly
|Hines WC,Yaswen P,Bissell MJ||Nature communications (6:null)||2015|
Nuclear shape changes are induced by knockdown of the SWI/SNF ATPase BRG1 and are independent of cytoskeletal connections.
MA1-12589 was used in immunocytochemistry to investigate the role of SWI/SNF ATPase BRG1 in nuclear morphology
|Imbalzano KM,Cohet N,Wu Q,Underwood JM,Imbalzano AN,Nickerson JA||PloS one (8:null)||2013|
|Not Applicable||Not Cited||
An oestrogen-dependent model of breast cancer created by transformation of normal human mammary epithelial cells.
MA1-12589 was used in immunocytochemistry and immunohistochemistry to develop an estrogen receptor-alpha-positive model of breast cancer using human mammary epithelial cells
|Duss S,André S,Nicoulaz AL,Fiche M,Bonnefoi H,Brisken C,Iggo RD||Breast cancer research : BCR (9:null)||2007|
Multiple oncocytic cystadenoma with intraluminal crystalloids in parotid gland: case report.
MA1-12589 was used in immunohistochemistry to describe a tumor from a patient with oncocytic cystadenoma
|Ba¿ak K,Kiro¿lu K||Medicine (93:null)||2014|
Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature.
MA1-12589 was used in immunohistochemistry to report on two cases of malignant transformation of adenomyoepithelioma of the breast by a monophasic population
|Marian C,Boila A,Soanca D,Malau M,Podeanu DM,Resetkova E,Stolnicu S||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (121:272)||2013|
Expression of KRT7 and WT1 differentiates precursor lesions of Wilms' tumours from those of papillary renal cell tumours and mucinous tubular and spindle cell carcinomas.
MA1-12589 was used in immunohistochemistry to study the value of KRT7 and WT1 expression profiles in differentiating Wilms' tumour, papillary renal cell tumour and mucinous tubular and spindle cell carcinoma precursor lesions
|Szponar A,Kovacs G||Virchows Archiv : an international journal of pathology (460:423)||2012|
Giant clear cell hidradenoma of the knee.
MA1-12589 was used in immunohistochemistry to report on a case of giant clear cell hidradenoma of the knee
|Yu G,Goodloe S,D'Angelis CA,McGrath BE,Chen F||Journal of cutaneous pathology (37:e37)||2010|
Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype.
MA1-12589 was used in immunohistochemistry to study 3D tumor spheroids enriched for a cancer stem cell phenotype
|Robertson FM,Ogasawara MA,Ye Z,Chu K,Pickei R,Debeb BG,Woodward WA,Hittelman WN,Cristofanilli M,Barsky SH||Journal of biomolecular screening (15:820)||2010|
|Non-human primate||Not Cited||
Endometrial profile of tamoxifen and low-dose estradiol combination therapy.
MA1-12589 was used in immunohistochemistry to detect effects of estradiol and tamoxifen on endometrial health
|Wood CE,Kaplan JR,Fontenot MB,Williams JK,Cline JM||Clinical cancer research : an official journal of the American Association for Cancer Research (16:946)||2010|
Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation.
MA1-12589 was used in immunohistochemistry to study malignant transformation in long-term cultures of bone marrow-derived human mesenchymal stem cells
|Røsland GV,Svendsen A,Torsvik A,Sobala E,McCormack E,Immervoll H,Mysliwietz J,Tonn JC,Goldbrunner R,Lønning PE,Bjerkvig R,Schichor C||Cancer research (69:5331)||2009|
Mammary gland development in early pubertal female macaques.
MA1-12589 was used in immunohistochemistry to investigate the effect of estradiol on early ductal and lobular development
|Wood CE,Hester JM,Cline JM||Toxicologic pathology (35:795)||2007|
An immunohistochemical profile of giant cell carcinoma of the larynx.
MA1-12589 was used in immunohistochemistry to report on a case of giant cell carcinoma of the larynx
|Gurbuz Y,Kose N,Aydin O,Ozturk M||Auris, nasus, larynx (34:413)||2007|
Cellular and molecular mechanisms of abnormal calcification following ischemia-reperfusion injury in human liver transplantation.
MA1-12589 was used in immunohistochemistry to investigate the mechanism for liver calcification after transplantation
|Kalantari F,Miao D,Emadali A,Tzimas GN,Goltzman D,Vali H,Chevet E,Auguste P||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (20:357)||2007|
Cytokeratin expression in lichen amyloidosus and macular amyloidosis.
MA1-12589 was used in immunohistochemistry to investigate the expression of cytokeratins in lichen and macular amyloidosis and their diagnostic value
|Apaydin R,Gürbüz Y,Bayramgürler D,Müezzinoglu B,Bilen N||Journal of the European Academy of Dermatology and Venereology : JEADV (18:305)||2004|
Alterations of canalicular ATP-binding cassette transporter expression in drug-induced liver injury.
MA1-12589 was used in immunohistochemistry - paraffin section to evaluate the role of canalicular ATP-binding cassette transporter expression in drug-induced liver injury
|Zollner G,Thueringer A,Lackner C,Fickert P,Trauner M||Digestion (90:81)||2014|
A small molecule inhibitor of SRC family kinases promotes simple epithelial differentiation of human pluripotent stem cells.
MA1-12589 was used in flow cytometry, immunocytochemistry, and western blot to describe conditions that promote development of hPSC-derived simple epithelial cells
|Lian X,Selekman J,Bao X,Hsiao C,Zhu K,Palecek SP||PloS one (8:null)||2013|
Directed differentiation of human embryonic stem cells to epidermal progenitors.
MA1-12589 was used in flow cytometry to discus a method for the differentiation of human embryonic stem cells into epidermal progenitors
|Metallo CM,Ji L,de Pablo JJ,Palecek SP||Methods in molecular biology (Clifton, N.J.) (585:83)||2009|
Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation.
MA1-12589 was used in immunoprecipitation to study the opposing effects of hyperglycemia and SIRT2 on keratin-8 lysine acetylation
|Snider NT,Leonard JM,Kwan R,Griggs NW,Rui L,Omary MB||The Journal of cell biology (200:241)||2013|
Monitoring of epithelial cell caspase activation via detection of durable keratin fragment formation.
MA1-12589 was used in ELISA to study epithelial cell caspase activation via detection of a durable keratin fragment
|Tao GZ,Li DH,Zhou Q,Toivola DM,Strnad P,Sandesara N,Cheung RC,Hong A,Omary MB||The Journal of pathology (215:164)||2008|