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Immunofluorescent analysis of Cytokeratin 18 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 18 (DC10) Mouse Monoclonal Antibody (MA5-12104) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Primate, Human, Mouse, Rhesus monkey|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human breast cancer PMC 42 cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunohistochemistry (IHC)||See 11 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Western Blot (WB)||See 11 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|ELISA (ELISA)||See 1 publications below|
MA5-12104 targets Cytokeratin 18 in IF, IHC (P), FACS, and WB applications and shows reactivity with Human samples.
The MA5-12104 immunogen is human breast cancer PMC 42 cells.
keratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins exists in combination with keratin 8. It was reported that tissues from gastrointestinal tract are positive for both keratin 8 and 18 but do not contain keratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for keratin 18.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Alterations of canalicular ATP-binding cassette transporter expression in drug-induced liver injury.
MA5-12104 was used in immunohistochemistry - paraffin section to evaluate the role of canalicular ATP-binding cassette transporter expression in drug-induced liver injury
|Zollner G,Thueringer A,Lackner C,Fickert P,Trauner M||Digestion (90:81)||2014|
Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature.
MA5-12104 was used in immunohistochemistry to report on two cases of malignant transformation of adenomyoepithelioma of the breast by a monophasic population
|Marian C,Boila A,Soanca D,Malau M,Podeanu DM,Resetkova E,Stolnicu S||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (121:272)||2013|
Expression of KRT7 and WT1 differentiates precursor lesions of Wilms' tumours from those of papillary renal cell tumours and mucinous tubular and spindle cell carcinomas.
MA5-12104 was used in immunohistochemistry to study the value of KRT7 and WT1 expression profiles in differentiating Wilms' tumour, papillary renal cell tumour and mucinous tubular and spindle cell carcinoma precursor lesions
|Szponar A,Kovacs G||Virchows Archiv : an international journal of pathology (460:423)||2012|
Giant clear cell hidradenoma of the knee.
MA5-12104 was used in immunohistochemistry to report on a case of giant clear cell hidradenoma of the knee
|Yu G,Goodloe S,D'Angelis CA,McGrath BE,Chen F||Journal of cutaneous pathology (37:e37)||2010|
Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype.
MA5-12104 was used in immunohistochemistry to study 3D tumor spheroids enriched for a cancer stem cell phenotype
|Robertson FM,Ogasawara MA,Ye Z,Chu K,Pickei R,Debeb BG,Woodward WA,Hittelman WN,Cristofanilli M,Barsky SH||Journal of biomolecular screening (15:820)||2010|
Endometrial profile of tamoxifen and low-dose estradiol combination therapy.
MA5-12104 was used in immunohistochemistry to detect effects of estradiol and tamoxifen on endometrial health
|Wood CE,Kaplan JR,Fontenot MB,Williams JK,Cline JM||Clinical cancer research : an official journal of the American Association for Cancer Research (16:946)||2010|
Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation.
MA5-12104 was used in immunohistochemistry to study malignant transformation in long-term cultures of bone marrow-derived human mesenchymal stem cells
|Røsland GV,Svendsen A,Torsvik A,Sobala E,McCormack E,Immervoll H,Mysliwietz J,Tonn JC,Goldbrunner R,Lønning PE,Bjerkvig R,Schichor C||Cancer research (69:5331)||2009|
Antigen expression of human eccrine sweat glands.
MA5-12104 was used in immunohistochemistry to identify the phenotypic markers of human eccrine sweat glands
|Li HH,Zhou G,Fu XB,Zhang L||Journal of cutaneous pathology (36:318)||2009|
Mammary gland development in early pubertal female macaques.
MA5-12104 was used in immunohistochemistry to investigate the effect of estradiol on early ductal and lobular development
|Wood CE,Hester JM,Cline JM||Toxicologic pathology (35:795)||2007|
An immunohistochemical profile of giant cell carcinoma of the larynx.
MA5-12104 was used in immunohistochemistry to report on a case of giant cell carcinoma of the larynx
|Gurbuz Y,Kose N,Aydin O,Ozturk M||Auris, nasus, larynx (34:413)||2007|
Cellular and molecular mechanisms of abnormal calcification following ischemia-reperfusion injury in human liver transplantation.
MA5-12104 was used in immunohistochemistry to investigate the mechanism for liver calcification after transplantation
|Kalantari F,Miao D,Emadali A,Tzimas GN,Goltzman D,Vali H,Chevet E,Auguste P||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (20:357)||2007|
Cytokeratin expression in lichen amyloidosus and macular amyloidosis.
MA5-12104 was used in immunohistochemistry to investigate the expression of cytokeratins in lichen and macular amyloidosis and their diagnostic value
|Apaydin R,Gürbüz Y,Bayramgürler D,Müezzinoglu B,Bilen N||Journal of the European Academy of Dermatology and Venereology : JEADV (18:305)||2004|
Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation.
MA5-12104 was used in immunoprecipitation to study the opposing effects of hyperglycemia and SIRT2 on keratin-8 lysine acetylation
|Snider NT,Leonard JM,Kwan R,Griggs NW,Rui L,Omary MB||The Journal of cell biology (200:241)||2013|
Antagonistic roles of Notch and p63 in controlling mammary epithelial cell fates.
MA5-12104 was used in western blot to investigate the role of Notch and p63 in regulation of mammary epithelial cell fates
|Yalcin-Ozuysal O,Fiche M,Guitierrez M,Wagner KU,Raffoul W,Brisken C||Cell death and differentiation (17:1600)||2010|
Cytoskeletal keratin glycosylation protects epithelial tissue from injury.
MA5-12104 was used in western blot to study the role of cytoskeletal keratin glycosylation in protecting epithelial tissue from injury
|Ku NO,Toivola DM,Strnad P,Omary MB||Nature cell biology (12:876)||2010|
Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.
MA5-12104 was used in western blot to study the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines
|Chang CY,Chiou PP,Chen WJ,Li YH,Yiu JC,Cheng YH,Chen SD,Lin CT,Lai YS||Research in veterinary science (88:285)||2010|
Frzb, a secreted Wnt antagonist, decreases growth and invasiveness of fibrosarcoma cells associated with inhibition of Met signaling.
MA5-12104 was used in western blot to study the effect of the secreted Wnt antagonist Frzb on fibrosarcoma growth and metastasis
|Guo Y,Xie J,Rubin E,Tang YX,Lin F,Zi X,Hoang BH||Cancer research (68:3350)||2008|
Analysis of keratin polypeptides 8 and 19 variants in inflammatory bowel disease.
MA5-12104 was used in western blot to study whether variants of keratin 8 and keratin 19 are associated with inflammatory bowel disease
|Tao GZ,Strnad P,Zhou Q,Kamal A,Zhang L,Madani ND,Kugathasan S,Brant SR,Cho JH,Omary MB,Duerr RH||Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association (5:857)||2007|
Protein phosphatase-2A associates with and dephosphorylates keratin 8 after hyposmotic stress in a site- and cell-specific manner.
MA5-12104 was used in western blot to study the site-specific dephosphorylation of keratin 8 by protein phosphatase-2A and how this varies according to cell type
|Tao GZ,Toivola DM,Zhou Q,Strnad P,Xu B,Michie SA,Omary MB||Journal of cell science (119:1425)||2006|
Identification of 14-3-3sigma as a contributor to drug resistance in human breast cancer cells using functional proteomic analysis.
MA5-12104 was used in western blot to study the role of 14-3-3sigma in drug resistance in human breast cancer cells
|Liu Y,Liu H,Han B,Zhang JT||Cancer research (66:3248)||2006|
Expression of Frzb/secreted Frizzled-related protein 3, a secreted Wnt antagonist, in human androgen-independent prostate cancer PC-3 cells suppresses tumor growth and cellular invasiveness.
MA5-12104 was used in western blot to investigate the suppressive effects of Frzb/sFRP3 expression on tumor growth and cellular invasiveness in PC-3 cells
|Zi X,Guo Y,Simoneau AR,Hope C,Xie J,Holcombe RF,Hoang BH||Cancer research (65:9762)||2005|
Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease.
MA5-12104 was used in western blot to study keratin 8 and keratin 18 hyperphosphorylation as markers for progression of human liver disease
|Toivola DM,Ku NO,Resurreccion EZ,Nelson DR,Wright TL,Omary MB||Hepatology (Baltimore, Md.) (40:459)||2004|
Keratin mutation in transgenic mice predisposes to Fas but not TNF-induced apoptosis and massive liver injury.
MA5-12104 was used in western blot to study the role of keratin mutations in the predisposition to Fas-induced apoptosis and massive liver injury
|Ku NO,Soetikno RM,Omary MB||Hepatology (Baltimore, Md.) (37:1006)||2003|
Keratin 8 mutations in patients with cryptogenic liver disease.
MA5-12104 was used in western blot to study mutations of keratin 8 in cryptogenic liver disease patients
|Ku NO,Gish R,Wright TL,Omary MB||The New England journal of medicine (344:1580)||2001|
Directed differentiation of human embryonic stem cells to epidermal progenitors.
MA5-12104 was used in flow cytometry to discus a method for the differentiation of human embryonic stem cells into epidermal progenitors
|Metallo CM,Ji L,de Pablo JJ,Palecek SP||Methods in molecular biology (Clifton, N.J.) (585:83)||2009|
Monitoring of epithelial cell caspase activation via detection of durable keratin fragment formation.
MA5-12104 was used in ELISA to study epithelial cell caspase activation via detection of a durable keratin fragment
|Tao GZ,Li DH,Zhou Q,Toivola DM,Strnad P,Sandesara N,Cheung RC,Hong A,Omary MB||The Journal of pathology (215:164)||2008|
cell proliferation-inducing gene 46 protein; cell proliferation-inducing protein 46; CK-18; cytokeratin 18; cytokeratin endo B; cytokeratin-18; Endo B; keratin; keratin 18, type I; keratin complex 1, acidic, gene 18; keratin D; keratin, type I cytoskeletal 18; keratin-18; type I cytoskeletal 18
CK-18; CK18; CYK18; K18; Kerd; Krt1-18; KRT18; PIG46