|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (commercially sensitive amino acid sequence) C-terminal (Human).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-21060 detects Cytokeratin 19 in Human samples.
PA1-21060 has been successfully used in Immunofluorescence and Immunohistochemistry (Paraffin) procedures. Staining of formalin fixed tissues requires digesting tissue sections in pepsin for 10 minutes at 37°C.
PA1-21060 immunogen corresponds to synthetic peptide (commercially sensitive amino acid sequence) C terminal (Human).
This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.