Immunofluorescence analysis of Cytokeratin 5 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 5 (XM26) Mouse Monoclonal Antibody (Product # MA5-12596) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant protein corresponding to C-terminal 103 aa of cytokeratin 5|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12596 targets Cytokeratin 5 in IHC (P) applications and shows reactivity with Human samples.
The MA5-12596 immunogen is recombinant protein corresponding to C-terminal 103 aa of cytokeratin 5.
Twenty human keratins are divided into acidic (pI <5.7) and basic (pI >6.0) subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor identification. Point mutations in cytokeratin5 gene can cause various types of epidermolysis bullosa simplex. It is expressed in most epithelial and biphasic mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Steroid induction of therapy-resistant cytokeratin-5-positive cells in estrogen receptor-positive breast cancer through a BCL6-dependent mechanism.
MA5-12596 was used in immunocytochemistry to use of estrogen receptor-positive breast cancer lines to study steroid induction of therapy-resistant cytokeratin-5-positive cells through a BCL6-dependent mechanism
|Goodman CR,Sato T,Peck AR,Girondo MA,Yang N,Liu C,Yanac AF,Kovatich AJ,Hooke JA,Shriver CD,Mitchell EP,Hyslop T,Rui H||Oncogene (35:1373)||2016|
Prolactin suppresses a progestin-induced CK5-positive cell population in luminal breast cancer through inhibition of progestin-driven BCL6 expression.
MA5-12596 was used in immunocytochemistry, immunohistochemistry, and western blot to study the role of BCL6 in the mechanism by which prolactin blocks the induction of a CK5 cell population by progestin in luminal breast cancer
|Sato T,Tran TH,Peck AR,Girondo MA,Liu C,Goodman CR,Neilson LM,Freydin B,Chervoneva I,Hyslop T,Kovatich AJ,Hooke JA,Shriver CD,Fuchs SY,Rui H||Oncogene (33:2215)||2014|
Targeted Pten deletion plus p53-R270H mutation in mouse mammary epithelium induces aggressive claudin-low and basal-like breast cancer.
MA5-12596 was used in immunohistochemistry - paraffin section to study induction of aggressive claudin-low and basal-like breast cancer by targeted Pten deletion plus p53-R270H mutation in mouse mammary epithelium
|Wang S,Liu JC,Kim D,Datti A,Zacksenhaus E||Breast cancer research : BCR (18:null)||2016|
|Not Applicable||Not Cited||
High frequency of loss of PTEN expression in human solid salivary adenoid cystic carcinoma and its implication for targeted therapy.
MA5-12596 was used in immunohistochemistry - paraffin section to characterize human solid salivary adenoid cystic carcinoma and high frequency of loss of PTEN expression as a targeted therapy
|Liu H,Du L,Wang R,Wei C,Liu B,Zhu L,Liu P,Liu Q,Li J,Lu SL,Xiao J||Oncotarget (6:11477)||2015|
Biological characteristics and clinical outcome of triple negative primary breast cancer in older women - comparison with their younger counterparts.
MA5-12596 was used in immunohistochemistry - paraffin section to study triple negative primary breast cancer biological characteristics and clinical outcome in older women in comparison to their younger counterparts
|Syed BM,Green AR,Nolan CC,Morgan DA,Ellis IO,Cheung KL||PloS one (9:null)||2014|
Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors.
MA5-12596 was used in immunohistochemistry - paraffin section to characterize Brazilian breast cancer cell lines
|Corrêa NC,Kuasne H,Faria JA,Seixas CC,Santos IG,Abreu FB,Nonogaki S,Rocha RM,Aparecida Borges Silva G,Gobbi H,Rogatto SR,Goes AM,Gomes DA||Oncology reports (29:1299)||2013|
HIV nucleoside reverse transcriptase inhibitors efavirenz and tenofovir change the growth and differentiation of primary gingival epithelium.
MA5-12596 was used in immunohistochemistry to use organotypic gingival keratinocyte culture to explore the effects of HIV nucleoside reverse transcriptase inhibitors on the gingival epithelium
|Mitchell D,Israr M,Alam S,Dinello D,Kishel J,Jia R,Meyers C||HIV medicine (15:196)||2014|
A systematic analysis of commonly used antibodies in cancer diagnostics.
MA5-12596 was used in immunohistochemistry to perform a systematic study of the diagnostic power of a range of antibodies commonly used in the diagnosis of cancer
|Gremel G,Bergman J,Djureinovic D,Edqvist PH,Maindad V,Bharambe BM,Khan WA,Navani S,Elebro J,Jirström K,Hellberg D,Uhlén M,Micke P,Pontén F||Histopathology (64:293)||2014|
Tumor grade and matrix metalloproteinase 2 expression in stromal fibroblasts help to stratify the high-risk group of patients with early breast cancer identified on the basis of st Gallen recommendations.
MA5-12596 was used in immunohistochemistry to study the use of tumor grade plus stromal fibroblast MMP2 expression to help stratify high-risk early breast cancer patients
|Niemiec J,Adamczyk A,Ma¿ecki K,Ambicka A,Ry¿ J||Clinical breast cancer (13:119)||2013|
Effect of the HIV nucleoside reverse transcriptase inhibitor zidovudine on the growth and differentiation of primary gingival epithelium.
MA5-12596 was used in immunohistochemistry to investigate the influence of zidovudine on gingival epithelium growth
|Mitchell D,Israr M,Alam S,Kishel J,Dinello D,Meyers C||HIV medicine (13:276)||2012|
Identification of the receptor tyrosine kinase AXL in breast cancer as a target for the human miR-34a microRNA.
MA5-12596 was used in immunohistochemistry to identify the targets of miR-34a microRNA in human breast cancer
|Mackiewicz M,Huppi K,Pitt JJ,Dorsey TH,Ambs S,Caplen NJ||Breast cancer research and treatment (130:663)||2011|
Molecular phenotypes of matched in situ and invasive components of breast carcinomas.
MA5-12596 was used in immunohistochemistry to compare the tissue microarray immunoprofiles of in situ and invasive breast carcinoma components within the same sample
|Martins D,Sousa B,Lopes N,Gomes M,Veronese L,Albergaria A,Paredes J,Schmitt F||Human pathology (42:1438)||2011|
Two novel recessive mutations in KRT14 identified in a cohort of 21 Spanish families with epidermolysis bullosa simplex.
MA5-12596 was used in immunohistochemistry to study two novel recessive mutations of KRT14 in a cohort of 21 Spanish families with epidermolysis bullosa simplex
|García M,Santiago JL,Terrón A,Hernández-Martín A,Vicente A,Fortuny C,De Lucas R,López JC,Cuadrado-Corrales N,Holguín A,Illera N,Duarte B,Sánchez-Jimeno C,Llames S,García E,Ayuso C,Martínez-Santamaría L,Castiglia D,De Luca N,Torrelo A,Mechan D,Baty D,Zambruno G,Escámez MJ,Del Río M||The British journal of dermatology (165:683)||2011|
Tumor-in-tumor of the thyroid with basaloid differentiation: a lesion with a solid cell nest neoplastic component?
MA5-12596 was used in immunohistochemistry to report on a case of tumor-in-tumor of the thyroid with basaloid differentiation
|Eloy C,Vinagre J,Cameselle-Teijeiro J,Paiva ME,Soares P,Sobrinho-Simões M||International journal of surgical pathology (19:276)||2011|
Cell adhesion and communication proteins are differentially expressed in melanoma progression model.
MA5-12596 was used in immunohistochemistry to study the immunohistochemical expression of a number of adhesion and communication molecules in a melanoma progression model.
|Rezze GG,Fregnani JH,Duprat J,Landman G||Human pathology (42:409)||2011|
Increased NOS2 predicts poor survival in estrogen receptor-negative breast cancer patients.
MA5-12596 was used in immunohistochemistry to investigate the prognostic significance of inducible nitric oxide synthase in estrogen receptor-negative breast cancer
|Glynn SA,Boersma BJ,Dorsey TH,Yi M,Yfantis HG,Ridnour LA,Martin DN,Switzer CH,Hudson RS,Wink DA,Lee DH,Stephens RM,Ambs S||The Journal of clinical investigation (120:3843)||2010|
Molecular characterization of breast cancer in young Brazilian women.
MA5-12596 was used in immunohistochemistry to study the molecular features of breast cancer in young Brazilian women
|Carvalho LV,Pereira EM,Frappart L,Boniol M,Bernardo WM,Tarricone V,Tavtigian S,Southey MC||Revista da Associacao Medica Brasileira (1992) (56:278)||2010|
Cytological criteria to predict basal phenotype of breast carcinomas.
MA5-12596 was used in immunohistochemistry to investigate the phenotypic classification of breast cancer based on cytological criteria
|Dufloth RM,Alves JM,Martins D,Vieira DS,Chikota H,Zeferino LC,Schmitt F||Diagnostic cytopathology (37:809)||2009|
Expression and prognostic significance of metalloproteases and their inhibitors in luminal A and basal-like phenotypes of breast carcinoma.
MA5-12596 was used in immunohistochemistry to investigate the importance of matrix metalloproteases and tissue inhibitors in certain types of breast carcinoma
|González LO,Corte MD,Junquera S,González-Fernández R,del Casar JM,García C,Andicoechea A,Vázquez J,Pérez-Fernández R,Vizoso FJ||Human pathology (40:1224)||2009|
Basal phenotype in breast carcinoma occurring in women aged 35 or younger.
MA5-12596 was used in immunohistochemistry to study the basal phenotype of breast carcinoma occuring in young women
|Bori R,Cserni G||Pathology oncology research : POR (15:41)||2009|
Differences in the tumor microenvironment between African-American and European-American breast cancer patients.
MA5-12596 was used in immunohistochemistry to compare tumor microenvironments between African-American and European-American breast cancer patients
|Martin DN,Boersma BJ,Yi M,Reimers M,Howe TM,Yfantis HG,Tsai YC,Williams EH,Lee DH,Stephens RM,Weissman AM,Ambs S||PloS one (4:null)||2009|
Identification of molecular phenotypes in canine mammary carcinomas with clinical implications: application of the human classification.
MA5-12596 was used in immunohistochemistry to evaluate the canine mammary carcinomas as cognate human cancer models
|Gama A,Alves A,Schmitt F||Virchows Archiv : an international journal of pathology (453:123)||2008|
Keratin profiling in the developing human prostate. A different approach to understanding epithelial lineage.
MA5-12596 was used in immunohistochemistry to investigate the distribution of keratin in prostate during development
|Trompetter M,Smedts F,van der Wijk J,Schoots C,de Jong HJ,Hopman A,de la Rosette J||Anticancer research (28:237)||2008|
|Not Applicable||Not Cited||
Adenosquamous carcinoma with cilium formation, mucin production and keratinization in the nasal cavity of a red fox (Vulpes vulpes schrencki).
MA5-12596 was used in immunohistochemistry to report on a case of adenosquamous carcinoma in the nasal cavity of a red fox
|Fukui D,Bando G,Ishikawa Y,Kadota K||Journal of comparative pathology (137:142)||2007|
Tissue microarrays for testing basal biomarkers in familial breast cancer cases.
MA5-12596 was used in immunohistochemistry to study the use of tissue microarray technology for studying the biomarker profile in familial beast cancer
|Dufloth RM,Matos I,Schmitt F,Zeferino LC||Sao Paulo medical journal = Revista paulista de medicina (125:226)||2007|
Nestin is expressed in the basal/myoepithelial layer of the mammary gland and is a selective marker of basal epithelial breast tumors.
MA5-12596 was used in immunohistochemistry to study nestin expression as a selective marker of basal epithelial breast tumors
|Li H,Cherukuri P,Li N,Cowling V,Spinella M,Cole M,Godwin AK,Wells W,DiRenzo J||Cancer research (67:501)||2007|
P-cadherin and cytokeratin 5: useful adjunct markers to distinguish basal-like ductal carcinomas in situ.
MA5-12596 was used in immunohistochemistry to investigate the expression of p-cadherin and cytokeratin 5 in basal-like ductal carcinomas in situ
|Paredes J,Lopes N,Milanezi F,Schmitt FC||Virchows Archiv : an international journal of pathology (450:73)||2007|
p63, cytokeratin 5, and P-cadherin: three molecular markers to distinguish basal phenotype in breast carcinomas.
MA5-12596 was used in immunohistochemistry to study the diagnostic markers of basal breast carcinomas
|Matos I,Dufloth R,Alvarenga M,Zeferino LC,Schmitt F||Virchows Archiv : an international journal of pathology (447:688)||2005|
Immunohistochemical profile of high-grade ductal carcinoma in situ of the breast.
MA5-12596 was used in immunoprecipitation to perform an immunohistochemical sub-typing of high-grade ductal carcinoma in situ of the breast
|Perez AA,Rocha RM,Balabram D,Souza Áda S,Gobbi H||Clinics (Sao Paulo, Brazil) (68:674)||2013|