|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||A synthetic peptide (GSSTIKYTTTS) from the C-terminus of human keratin 6|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Functional Assay (FN)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14127 targets Cytokeratin 6 in FN applications and shows reactivity with Human and mouse samples.
The MA5-14127 immunogen is a synthetic peptide (GSSTIKYTTTS) from the C-terminus of human keratin 6.
Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HIV nucleoside reverse transcriptase inhibitors efavirenz and tenofovir change the growth and differentiation of primary gingival epithelium.
MA5-14127 was used in immunohistochemistry to use organotypic gingival keratinocyte culture to explore the effects of HIV nucleoside reverse transcriptase inhibitors on the gingival epithelium
|Mitchell D,Israr M,Alam S,Dinello D,Kishel J,Jia R,Meyers C||HIV medicine (15:196)||2014|
Keratin 79 identifies a novel population of migratory epithelial cells that initiates hair canal morphogenesis and regeneration.
MA5-14127 was used in immunohistochemistry to study the role of novel keratin 79-positive migratory epithelial cells in hair canal morphogenesis and regeneration
|Veniaminova NA,Vagnozzi AN,Kopinke D,Do TT,Murtaugh LC,Maillard I,Dlugosz AA,Reiter JF,Wong SY||Development (Cambridge, England) (140:4870)||2013|
Keratin gene expression profiles after digit amputation in C57BL/6 vs. regenerative MRL mice imply an early regenerative keratinocyte activated-like state.
MA5-14127 was used in immunohistochemistry to study the expression pattern of keratins in C57BL/6 mice and MRL regenerative mice before and after amputation of the middle digit
|Cheng CH,Leferovich J,Zhang XM,Bedelbaeva K,Gourevitch D,Hatcher CJ,Basson CT,Heber-Katz E,Marx KA||Physiological genomics (45:409)||2013|
Bioengineered skin humanized model of psoriasis.
MA5-14127 was used in immunohistochemistry to use bioengineering techniques to develop a murine skin humanized psoriasis model
|Carretero M,Guerrero-Aspizua S,Del Río M||Methods in molecular biology (Clifton, N.J.) (961:305)||2013|
Effect of the HIV nucleoside reverse transcriptase inhibitor zidovudine on the growth and differentiation of primary gingival epithelium.
MA5-14127 was used in immunohistochemistry to investigate the influence of zidovudine on gingival epithelium growth
|Mitchell D,Israr M,Alam S,Kishel J,Dinello D,Meyers C||HIV medicine (13:276)||2012|
Development of skin-humanized mouse models of pachyonychia congenita.
MA5-14127 was used in immunohistochemistry to develop and evaluate a mouse model for pachyonychia congenita
|García M,Larcher F,Hickerson RP,Baselga E,Leachman SA,Kaspar RL,Del Rio M||The Journal of investigative dermatology (131:1053)||2011|
Down-regulation of keratin 4 and keratin 13 expression in oral squamous cell carcinoma and epithelial dysplasia: a clue for histopathogenesis.
MA5-14127 was used in immunohistochemistry to study the expression of keratin 4 and keratin 13 in oral squamous cell carcinoma and epithelial dysplasia
|Sakamoto K,Aragaki T,Morita K,Kawachi H,Kayamori K,Nakanishi S,Omura K,Miki Y,Okada N,Katsube K,Takizawa T,Yamaguchi A||Histopathology (58:531)||2011|
Multiple functions of Snail family genes during palate development in mice.
MA5-14127 was used in immunohistochemistry to study the multiple functions of Snail family genes during mouse palate development
|Murray SA,Oram KF,Gridley T||Development (Cambridge, England) (134:1789)||2007|
New skin-equivalent model from de-epithelialized amnion membrane.
MA5-14127 was used in immunohistochemistry to develop a living skin-equivalent from de-epithelialized amnion membrane
|Yang L,Shirakata Y,Shudou M,Dai X,Tokumaru S,Hirakawa S,Sayama K,Hamuro J,Hashimoto K||Cell and tissue research (326:69)||2006|
Characterization of medroxyprogesterone and DMBA-induced multilineage mammary tumors by gene expression profiling.
MA5-14127 was used in immunohistochemistry to study the gene expression profile of medroxyprogesterone- and DMBA-induced multilineage mammary tumors
|Yin Y,Bai R,Russell RG,Beildeck ME,Xie Z,Kopelovich L,Glazer RI||Molecular carcinogenesis (44:42)||2005|
A study of cytokeratin profiles in localized cutaneous amyloids.
MA5-14127 was used in immunohistochemistry to investigate the expression profile of cytokeratins in cutaneous amyloids
|Chang YT,Liu HN,Wang WJ,Lee DD,Tsai SF||Archives of dermatological research (296:83)||2004|
Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus.
MA5-14127 was used in immunohistochemistry to study the regulation of epithelial cell differentiation and human thymic medulla Hassall body formation by corticosteroids
|Hale LP,Markert ML||Journal of immunology (Baltimore, Md. : 1950) (172:617)||2004|
Toward a molecular pathologic classification of urothelial carcinoma.
MA5-14127 was used in immunohistochemistry (paraffin) to describe the molecular pathologic characteristics of urothelial carcinomas subtypes.
|Sjödahl G,Lövgren K,Lauss M,Patschan O,Gudjonsson S,Chebil G,Aine M,Eriksson P,Månsson W,Lindgren D,Fernö M,Liedberg F,Höglund M||The American journal of pathology (183:681)||2013|
Epidermal growth factor-induced modulation of cytokeratin expression levels influences the morphological phenotype of head and neck squamous cell carcinoma cells.
MA5-14127 was used in western blot to study the effect on the morphology of head and neck squamous cell carcinoma cells of EGF-mediated regulation of cytokeratin expression
|Makarova G,Bette M,Schmidt A,Jacob R,Cai C,Rodepeter F,Betz T,Sitterberg J,Bakowsky U,Moll R,Neff A,Sesterhenn A,Teymoortash A,Ocker M,Werner JA,Mandic R||Cell and tissue research (351:59)||2013|
Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.
MA5-14127 was used in blocking or activating experiment to study the role of Ku70/80 and DNA-PK in regulating RAG-mediated cleavage and the significance for the 12/23 rule specificity
|Sawchuk DJ,Mansilla-Soto J,Alarcon C,Singha NC,Langen H,Bianchi ME,Lees-Miller SP,Nussenzweig MC,Cortes P||The Journal of biological chemistry (279:29821)||2004|