Immunofluorescent analysis of Cytokeratin Pan (green) showing staining in the cytoplasm of MCF-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytokeratin Pan monoclonal antibody (Product # MA5-13203) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Fish, Mouse, Human|
|Host / Isotype||Mouse / IgG|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 ug/ml|
|Western Blot (WB)||1:1000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is a broad-spectrum pan-keratin monoclonal antibody cocktail which recognizes keratin 4, 5, 6, 7, 8, 10, 13, 14, 18 and 19. This product can differentiate between epithelial and non-epithelial tumors.
For staining of formalin-fixed tissue,digest sections wtih trypsin at 1mg/ml in PBS, 10min at 37°C, or Protease XXV at 1mg/ml in PBS for 5min at 37°C.
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Efficient Generation of Mice with Consistent Transgene Expression by FEEST.
MA5-13203 was used in immunohistochemistry - paraffin section to describe functionally enriched ES cell transgenics
|Gao L,Jiang Y,Mu L,Liu Y,Wang F,Wang P,Zhang A,Tang N,Chen T,Luo M,Yu L,Gao S,Chen L||Scientific reports (5:null)||2015|
Regulation of the inflammatory profile of stromal cells in human breast cancer: prominent roles for TNF-¿ and the NF-¿B pathway.
MA5-13203 was used in western blot to study the impact of TNF-alpha and IL-1beta on the inflammatory phenotype of cancer-associated fibroblasts and mesenchymal stem/stromal cells
|Katanov C,Lerrer S,Liubomirski Y,Leider-Trejo L,Meshel T,Bar J,Feniger-Barish R,Kamer I,Soria-Artzi G,Kahani H,Banerjee D,Ben-Baruch A||Stem cell research and therapy (6:null)||2015|
Fine needle aspiration cytology of inflammatory myofibroblastic tumour (inflammatory pseudotumour) of the breast: a case report and review of the literature.
MA5-13203 was used in immunohistochemistry to investigate a clinical case of inflammatory myofibroblastic tumour in the breast
|Akbulut M,Gunhan-Bilgen I,Zekioglu O,Duygulu G,Oktay A,Ozdemir N||Cytopathology : official journal of the British Society for Clinical Cytology (18:384)||2007|
Epithelioid leiomyosarcoma with rhabdoid features.
MA5-13203 was used in immunohistochemistry to report on a case of epithelioid leiomyosarcoma with rhabdoid features
|Yorulmaz G,Erdogan G,Pestereli HE,Savas B,Karaveli FS||Wiener klinische Wochenschrift (119:557)||2007|
Incidental calcifying fibrous tumor of the stomach presenting as a polyp.
MA5-13203 was used in immunohistochemistry to report on a case of incidental calcifying fibrous tumor of the stomach presenting as a polyp
|Elpek GO,Küpesiz GY,Ogüs M||Pathology international (56:227)||2006|
Mediastinal intraoperative radioisotope sentinel lymph node mapping in non-small-cell lung cancer.
MA5-13203 was used in immunohistochemistry to develop a method to map sentinel lymph nodes in non-small cell lung cancer patients
|Atinkaya C,Ozlem Küçük N,Koparal H,Aras G,Sak SD,Ozdemir N||Nuclear medicine communications (26:717)||2005|
MA5-13203 was used in immunohistochemistry to report on a case of anaplastic ependymoma.
|Erdo¿an G,Ozel E,Pe¿tereli HE,Salar Z,Tirak B,Karaveli S||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (113:301)||2005|
Persistent infection of betanodavirus in a novel cell line derived from the brain tissue of barramundi Lates calcarifer.
MA5-13203 was used in immunocytochemistry to establish and characterize a novel cell line infected with betanodavirus
|Chi SC,Wu YC,Cheng TM||Diseases of aquatic organisms (65:91)||2005|
Efficacy of a recombinant chimeric anti-hCG antibody to prevent human cytotrophoblasts fusion and block progesterone synthesis.
MA5-13203 was used in immunocytochemistry to study the use of a recombinant chimeric anti-hCG antibody to neutralize the bioactivity of hCG on human trophoblasts
|Dhar R,Karmakar S,Sriraman R,Talwar GP,Das C||American journal of reproductive immunology (New York, N.Y. : 1989) (51:358)||2004|
Alteration of integrin expression relates to malignant progression of human papillomavirus-immortalized esophageal keratinocytes.
MA5-13203 was used in immunocytochemistry to study the role of the integrins in the malignant progression of human papillomavirus-immortalized esophageal keratinocytes
|Sashiyama H,Shino Y,Sakao S,Shimada H,Kobayashi S,Ochiai T,Shirasawa H||Cancer letters (177:21)||2002|