|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human DLK1 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/200 - 1/400|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
MA5-15915 targets DLK1 in FACS, IF, and WB applications and shows reactivity with Human samples.
The MA5-15915 immunogen is purified recombinant fragment of human DLK1 expressed in E. Coli. .
MA5-15915 detects DLK1 which has a predicted molecular weight of approximately 41kDa.
This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Regulation of fibroblast lipid storage and myofibroblast phenotypes during alveolar septation in mice.
MA5-15915 was used in flow cytometry to examine pdgfralpha gene expression in mesenchymal progenitors and differentiation in lung fibroblasts
|McGowan SE,McCoy DM||American journal of physiology. Lung cellular and molecular physiology (307:L618)||2014|