Invitrogen

DYKDDDDK Tag Monoclonal Antibody (FG4R), DyLight™ 550

Product Image Gallery

DYKDDDDK Tag Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of DYKDDDDK Tag was performed using 70% confluent HA-H3-DYKDDDDK transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DYKDDDDK Tag Monoclonal Antibody (FG4R), DyLight 550 (Product # MA1-91878-D550) at 5 µg/mL and Histone H3 Recombinant Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2,000 for 45 minutes at room temperature. Panel a (Nuclei:Red) represents DYKDDDDK Tag. Panel b (Nuclei:Green) represents Histone H3. Panel c (Nuclei: Blue) represents ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel d represents the merged image showing the co-localization of nuclear signals in transfected cells. Panel e represents untransfected HEK-293 cells. Panel f represents control cells with No primary antibody control to assess background. The images were captured at 60X magnification.

DYKDDDDK Tag Antibody in Western Blot (WB) — Published figure using DYKDDDDK Tag monoclonal antibody (Product # MA1-91878-D550) in Western Blot

DYKDDDDK Tag Antibody in ELISA (ELISA) — Fig 4 Authentic RGD- and divalent cation-dependent binding of FMDV empty capsids to truncated, FLAG-tagged bovine alphavbeta6. (A) Sandwich ELISA for the detection of FMDV EC. Wells were coated with cell culture supernatant from mock-transfected cells (Mock) or cells co-transfected with expression plasmids for the alphav and beta6-FLAG subunits (alphavbeta6) and incubated with FMDV A22ecRGD (RGD) or A22ecKGA (KGA). FMDV capsid proteins were detected using an anti-A22 Iraq, guinea-pig polyclonal sera. (B) The same ELISA using purified alphavbeta6-FLAG in place of transfected cell supernatants. (C) Wells were coated with cell culture supernatant from mock-transfected cells (Mock) or cells co-transfected with both alphav and beta6-FLAG subunits (alphavbeta6) and incubated with FMDV A22ecRGD (ecRGD) in the presence or absence of an RGD-containing peptide, an inactive control RGE peptide or EDTA (as indicated on the figure). (D) An ELISA using truncated alphavbeta6-FLAG to detect FMDV empty capsid. Wells were coated with A22ecRGD (RGD), A22ecKGA (KGA) or coating buffer alone (CB) and capsid proteins detected using FLAG-tagged truncated alphavbeta6 (alphavbeta6) i.e. cell culture supernatant from alphavbeta6 co-transfected cells. RGD/Mock wells were coated with A22ecRGD and incubated with cell culture supernatant from mock-transfected cells.

Product Details

MA1-91878-D550

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	5 µg/mL	-
ELISA (ELISA)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Tag
Published species	Tag
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	FG4R
Immunogen	Synthetic peptide (DYKDDDDK) coupled to KLH.
Conjugate	DyLight™ 550 Biotin DyLight 488 DyLight 650 DyLight 680 DyLight 800 4X PEG HRP Unconjugated
Excitation/Emission Max	556/570 nm View spectra
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS with proprietary stabilizer
Contains	0.02% sodium azide
Storage conditions	4° C, do not freeze
RRID	AB_2537622

Product Specific Information

MA1-91878-D550 has been successfully used for immunofluorescence. DyLight 550 has an excitation/emission of 562/576 nm.

similar epitope as the FLAG tag from Sigma

Target Information

DYKDDDDK Tag (FLAG® tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Truncated Bovine Integrin Alpha-v/Beta-6 as a Universal Capture Ligand for FMD Diagnosis.

PloS one

Shimmon G,Wood BA,Morris A,Mioulet V,Grazioli S,Brocchi E,Berryman S,Tuthill T,King DP,Burman A,Jackson T

Published figure using DYKDDDDK Tag monoclonal antibody (Product # MA1-91878-D550) in ELISA

Thu Aug 03 00:00:00 EDT 2017

U2AF1 mutations alter splice site recognition in hematological malignancies.

Genome research

Ilagan JO,Ramakrishnan A,Hayes B,Murphy ME,Zebari AS,Bradley P,Bradley RK

Published figure using DYKDDDDK Tag monoclonal antibody (Product # MA1-91878-D550) in Western Blot

Thu Jan 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag

DYKDDDDK Tag Monoclonal Antibody (FG4R), DyLight™ 550

Product Details

MA1-91878-D550

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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