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Invitrogen
Desmosome/Hemidesmosome Chimeric Recombinant Rabbit Monoclonal Antibody (F12)
FIGURE: 1 / 1
Desmosome/Hemidesmosome Chimeric Antibody (MA5-48076) in Flow
Flow Cytometry analysis of Desmosome/Hemidesmosome in A431 cells using Desmosome/Hemidesmosome Chimeric Monoclonal Antibody (Product # MA5-48076). Cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with an isotype control (black line), or Desmosome/Hemidesmosome Chimeric Monoclonal Antibody (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detecte... View More
Product Details
MA5-48076
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
F12
Immunogen
F12 was established by fusing peripheral blood lymphocytes derived from a human vulgaris patient with a hetermyeloma cell line.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074519
Product Specific Information
Specificity: F12 recognizes an unknown polypeptide of desmosomal and hemidesmosomal plaques with a MW of 185 kDa (determined from immunoblotting using bovine tongue epithelium extract as the substrate), and by IEM (immunoelectron microscopy) it was determined that it is the intracellular part of the desmosome that is bound. F12 shares some immunochemical properties with autoantibodies present in paraneoplastic pemphigus sera. Pemphigus diseases are those resulting in blistering of the skin due to the production of autoantibodies against keratinocyte surface proteins-autoantibodies of patients with pemphius vulgaris recognize desmoglein 1 (Dsg1), a major glycoprotein of the desmosome complex.
Target Information
Desmosomes and Hemidesmosomes are two types of intercellular junctions that play important roles in maintaining the structural integrity of tissues subjected to mechanical stress or shear forces. Desmosomes connect neighboring cells using cadherins and intermediate filaments, while Hemidesmosomes anchor epithelial cells to the underlying extracellular matrix using integrins. Both junctions are found in tissues like skin, heart, and muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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