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|Tested species reactivity||Chemical|
|Published species reactivity||Chemical|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-0.5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|In Situ Hybridization (ISH) (ISH)||See 1 publications below|
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Digoxigenin (DIG), a steroid, is a derivative of the flowers and leaves of plants – Digitalis. Due to the high immunigenicity of this small molecule it is highly preferred in many molecular biology applications. DIG can be covalently added to proteins or nucleic acids which makes it very handy in its diverse applications. The digoxigenin markers are detected with anti-digoxigenin antibodies, which may be labeled with dyes or enzymes for visualization and detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Targeting epithelial-to-mesenchymal transition with Met inhibitors reverts chemoresistance in small cell lung cancer.
700772 was used in in situ hybridization to study the ability of Met inhibitors to reverse chemoresistance in small cell lung cancer and the role of epithelial-to-mesenchymal transition
|Cañadas I,Rojo F,Taus Á,Arpí O,Arumí-Uría M,Pijuan L,Menéndez S,Zazo S,Dómine M,Salido M,Mojal S,García de Herreros A,Rovira A,Albanell J,Arriola E||Clinical cancer research : an official journal of the American Association for Cancer Research (20:938)||2014|