|Tested species reactivity||Chemical|
|Published species reactivity||Chemical, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-0.5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Digoxigenin (DIG), a steroid, is a derivative of the flowers and leaves of plants – Digitalis. Due to the high immunigenicity of this small molecule it is highly preferred in many molecular biology applications. DIG can be covalently added to proteins or nucleic acids which makes it very handy in its diverse applications. The digoxigenin markers are detected with anti-digoxigenin antibodies, which may be labeled with dyes or enzymes for visualization and detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
High-Throughput Universal DNA Curtain Arrays for Single-Molecule Fluorescence Imaging.
700772 was used in ELISA to report a high-throughput method for DNA curtain studies
|Gallardo IF,Pasupathy P,Brown M,Manhart CM,Neikirk DP,Alani E,Finkelstein IJ||Langmuir : the ACS journal of surfaces and colloids (31:10310)||2015|
|Not Applicable||Not Cited||
Application of DNA Aptamers and Quantum Dots to Lateral Flow Test Strips for Detection of Foodborne Pathogens with Improved Sensitivity versus Colloidal Gold.
700772 was used in ELISA to report a proof-of-principle assay for the development of a highly sensitive aptamer-Qdot LF strip assay for rapid foodborne pathogen detection.
|Bruno JG||Pathogens (Basel, Switzerland) (3:341)||2014|
Isoform-specific expression of the neuropeptide orcokinin in Drosophila melanogaster.
700772 was used in immunocytochemistry to examine the distribution of orcokinin isoforms in Drosophilia
|Chen J,Choi MS,Mizoguchi A,Veenstra JA,Kang K,Kim YJ,Kwon JY||Peptides (68:50)||2015|
Targeting epithelial-to-mesenchymal transition with Met inhibitors reverts chemoresistance in small cell lung cancer.
700772 was used in in situ hybridization to study the ability of Met inhibitors to reverse chemoresistance in small cell lung cancer and the role of epithelial-to-mesenchymal transition
|Cañadas I,Rojo F,Taus Á,Arpí O,Arumí-Uría M,Pijuan L,Menéndez S,Zazo S,Dómine M,Salido M,Mojal S,García de Herreros A,Rovira A,Albanell J,Arriola E||Clinical cancer research : an official journal of the American Association for Cancer Research (20:938)||2014|