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Western blot analysis of Digoxin was performed by loading 10ng of BSA (Product # 23209) or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% milk in TBST for 1 hour at room temperature. BSA was detected at ~66kD using a BSA monoclonal antibody (Product # MA5-15238) at a dilution of 1ug/ml in 5% milk in TBST overnight at 4C on a rocking platform. Digoxin-labeled BSA was detected at ~66kD using a Digoxin monoclonal antibody (Product # MA5-14746) at a dilution of 1ug/ml in 5% milk in TBST overnight at 4C on a rocking platform. After washing, blots were probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate (Product # 34080).
|Tested species reactivity||Chem|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||0.01M potassium phosphate with 2.5mM KCl, 0.135M NaCl, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Radioimmune Assays (RIA)||Assay Dependent|
|Western Blot (WB)||1µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14746 targets Digoxin in Western Blot, ELISA, IP and RIA applications and shows reactivity with labeled oligonucleotides and chemical samples.
Product MA514746 is a smaller package size of MID0302 (formerly sold as a Seradyn product).
Digoxin, also known as digitalis, is a purified cardiac glycoside extracted from the foxglove plant, Digitalis lanata. Its corresponding aglycone is digoxigenin, and its acetyl derivative is acetyldigoxin. Digoxin is widely used in the treatment of various heart conditions, namely atrial fibrillation, atrial flutter and sometimes heart failure that cannot be controlled by other medication. Digoxin preparations are commonly marketed under the trade names Lanoxin, Digitek, and Lanoxicaps. Digoxin is also used as an affinity system for nonradioactive labeling and detection of proteins and nucleic acids.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.