Western blot of Drebrin in whole HeLa cell extracts (lane 1) and MCF7 cells (lane 2) using a Drebrin polyclonal antibody (Product # PA1-20222). Detection was performed using a Goat anti-Rabbit IgG-Alkaline Phosphatase and 5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium substrate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues 22-42 of human Drebrin.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20222 detects Drebrin in rat and human samples. This antibody is expected to cross-react with mouse (100%) and chicken (85%) due to sequence homology.
PA1-20222 has been successfully used in Western blot procedures.
The PA1-20222 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues 22-42 of human Drebrin.
The cortical cytoskeleton, a highly cross-linked submembranal F-actin filament network, plays an important role in cell proliferation, differentiation, migration, cell morphology and oncogenic transformation. Drebrin (developmentally-regulated brain protein) is a major neuronal F-actin binding protein involved in the control of actin dynamics and neuronal morphogenesis. Three major isoforms of drebrin, resulting from alternative splicing of a single gene, have been identified in the brain: the embryonic isoforms designated E1 and E2 and the adult or A isoform (95-120 kDa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.