|Tested species reactivity||Human, Non-human primate, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Dynorphin A: AA-Residues 1-17 (porcine)|
|Storage buffer||whole serum diluted in PBS with 0.1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1/100 - 1/1500|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Radioimmune Assays (RIA)||1/10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody binds to both dynorphin A 1-17 and 1-8 but not with other big dynorphin cleavage products such as dynorphin B, also known as rimorphin.
This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. Overnight incubation with primary antibody is recommended.
The immunogen used to produce PA1-84557 is from Dynorphin A 1-17: Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln (porcine).
Dynorphin A immunoreactive cells are found in the paraventricular and supraoptic nucleus and in hippocampal mossy fibers of rat brain following kainic acid administration and colchicine induced neurotoxicity. Dynorphins play a role in multiple physiologic processes including pain perception and responses to stress. They mimic and compete with a number of opiate drugs. Dysregulation of dynorphin A expression has been linked to progression of neurodegenerative conditions such as Alzheimerâ€™s disease and appears to play a significant role in the pathobiology of epilepsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.