Sandwich ELISA analysis using a ten point standard curve plotted with a full-length active recombinant EGF protein standard at concentrations ranging from 12.5 ng/mL to 0.3 pg/mL bound by anti-EGF capture antibody coated onto the plate. The standard was detected using a biotin-conjugated EGF recombinant rabbit oligoclonal antibody (Product # 710569) at a concentration of 2ug/mL. A non-linear regression analysis was performed (4 PL) and LOD and LOQ for the antibody pair was determined.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, feline, non-human primate and mouse based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Epidermal growth factor (EGF) is a growth factor which belongs to the protein kinase superfamily. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. When EGF binds to its receptor EGFR; it stimulates cell growth, proliferation and differentiation. Encoded protein acts as a potent mitogenic factor that plays several important roles. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Studies suggest the protein may also play important rol ein activating the NF-kappa-B signaling cascade.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.