Immunofluorescence analysis of EGFR was done on 90% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled EGFR (111.6+199.12+ F4+11E8) Mouse Monoclonal Antibody (MA513343) at 2ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1/IgG2a|
|Immunogen||Extracellular and cytoplasmic domains of recombinant human EGFR protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13343 targets Epidermal Growth Factor Receptor in IP and WB applications and shows reactivity with Human samples.
The MA5-13343 immunogen is extracellular and cytoplasmic domains of recombinant human EGFR protein.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
IP-MS enrichment of EGFR (LFQ intensity): EGFR was enriched 586-fold from A549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and EGFR antibody (Part No. MA5-13343). See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cancer cells exploit eIF4E2-directed synthesis of hypoxia response proteins to drive tumor progression.
MA5-13343 was used in western blot to study the role of eIF4E2 in the ability of tumors to survive and proliferate under conditions of hypoxia
|Uniacke J,Perera JK,Lachance G,Francisco CB,Lee S||Cancer research (74:1379)||2014|
An oxygen-regulated switch in the protein synthesis machinery.
MA5-13343 was used in western blot to study an oxygen-regulated translation initiation complex
|Uniacke J,Holterman CE,Lachance G,Franovic A,Jacob MD,Fabian MR,Payette J,Holcik M,Pause A,Lee S||Nature (486:126)||2012|
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
MA5-13343 was used in western blot to investigate the mechanism of angiogenesis and metastasis promoted by hypoxic tumor cells
|Park JE,Tan HS,Datta A,Lai RC,Zhang H,Meng W,Lim SK,Sze SK||Molecular and cellular proteomics : MCP (9:1085)||2010|
Structure-function analysis of nucleolin and ErbB receptors interactions.
MA5-13343 was used in western blot to investigate the interaction between nucleolin and ErbB receptors
|Farin K,Di Segni A,Mor A,Pinkas-Kramarski R||PloS one (4:null)||2009|
MMTV-EGF receptor transgene promotes preneoplastic conversion of multiple steroid hormone-responsive tissues.
MA5-13343 was used in western blot to investigate the function of epidermal growth factor receptor in the initiation of reproductive tissue tumor development
|Marozkina NV,Stiefel SM,Frierson HF,Parsons SJ||Journal of cellular biochemistry (103:2010)||2008|
|Not Applicable||Not Cited||
Sulindac metabolites inhibit epidermal growth factor receptor activation and expression.
MA5-13343 was used in western blot to test if sulindac metabolites block activation or expression of the epidermal growth factor receptor
|Pangburn HA,Kraus H,Ahnen DJ,Rice PL||Journal of carcinogenesis (4:null)||2005|
Surface charge and hydrophobicity determine ErbB2 binding to the Hsp90 chaperone complex.
MA5-13343 was used in western blot to study the role of surface charge and hydrophobicity in ErbB2 binding to the Hsp90 chaperone complex
|Xu W,Yuan X,Xiang Z,Mimnaugh E,Marcu M,Neckers L||Nature structural and molecular biology (12:120)||2005|
Neuregulin expression, function, and signaling in human ovarian cancer cells.
MA5-13343 was used in western blot to investigate the characteristics of neuregulin in human ovarian cancer cells
|Gilmour LM,Macleod KG,McCaig A,Sewell JM,Gullick WJ,Smyth JF,Langdon SP||Clinical cancer research : an official journal of the American Association for Cancer Research (8:3933)||2002|
Regulation of EGFR trafficking and cell signaling by Sprouty2 and MIG6 in lung cancer cells.
MA5-13343 was used in immunoprecipitation to study the role of Sprouty2 and MIG6 in modulating lung cancer cell EGFR trafficking and signaling
|Walsh AM,Lazzara MJ||Journal of cell science (126:4339)||2013|
Metastasis suppressor tetraspanin CD82/KAI1 regulates ubiquitylation of epidermal growth factor receptor.
MA5-13343 was used in immunoprecipitation to study the modulation of EGFR ubiquitinylation by the CD82 metastasis suppressor
|Odintsova E,van Niel G,Conjeaud H,Raposo G,Iwamoto R,Mekada E,Berditchevski F||The Journal of biological chemistry (288:26323)||2013|
Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK.
MA5-13343 was used in immunoprecipitation to investigate the mechanism of ERK autocrine activation in normal human keratinocytes
|Kansra S,Stoll SW,Johnson JL,Elder JT||Molecular biology of the cell (15:4299)||2004|
Facile chemical functionalization of proteins through intein-linked yeast display.
MA5-13343 was used in flow cytometry to develop an intein-based yeast display system to enable the simple chemical functionalization of proteins
|Marshall CJ,Agarwal N,Kalia J,Grosskopf VA,McGrath NA,Abbott NL,Raines RT,Shusta EV||Bioconjugate chemistry (24:1634)||2013|
Ubiquitin-dependent regulation of COPII coat size and function.
MA5-13343 was used in immunocytochemistry to study the modulation of COPII coat size and function by ubiquitin ligase CUL3-KLHL12
|Jin L,Pahuja KB,Wickliffe KE,Gorur A,Baumgärtel C,Schekman R,Rape M||Nature (482:495)||2012|
Characterization of a transplantable hormone-responsive human prostatic cancer xenograft TEN12 and its androgen-resistant sublines.
MA5-13343 was used in immunocytochemistry to investigate the effectiveness of Xenograft TEN12 and its sublines on the progression of prostatic cancer
|Harper ME,Goddard L,Smith C,Nicholson RI||The Prostate (58:13)||2004|
Epidermal growth factor ligand/receptor loop and downstream signaling activation pattern in completely resected nonsmall cell lung cancer.
MA5-13343 was used in immunohistochemistry to study EGF receptor pathway activation in completely resected non-small cell lung cancer
|Volante M,Saviozzi S,Rapa I,Ceppi P,Cappia S,Calogero R,Novello S,Borasio P,Papotti M,Scagliotti GV||Cancer (110:1321)||2007|
Racial disparity of epidermal growth factor receptor expression in prostate cancer.
MA5-13343 was used in immunohistochemistry to study racial differences in EGFR expression in prostate cancer
|Shuch B,Mikhail M,Satagopan J,Lee P,Yee H,Chang C,Cordon-Cardo C,Taneja SS,Osman I||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (22:4725)||2004|
In vitro chemoresistance and biomarker profiles are unique for histologic subtypes of epithelial ovarian cancer.
MA5-13343 was used in immunohistochemistry to study whether there is a relationship between histologic subtype of epithelial ovarian cancer and chemoresistance
|Cloven NG,Kyshtoobayeva A,Burger RA,Yu IR,Fruehauf JP||Gynecologic oncology (92:160)||2004|