Immunofluorescent analysis of EHMT2/G9a showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 5 min and stained using an EHMT2/G9a polyclonal antibody (Product # PA5-34971) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide contains a sequence corresponding to a region within amino acids 21 and 118 of G9a|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34971 targets EHMT2/G9a in ICC, IF, IHC (P), IP, and WB applications and shows reactivity with Human samples.
The PA5-34971 immunogen is synthetic peptide contains a sequence corresponding to a region within amino acids 21 and 118 of G9a.
A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described.
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