Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000-1:2,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:150 | - |
Flow Cytometry (Flow) |
1:100 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
OTI1F2 |
Immunogen |
Full length human recombinant protein of human EIF4E2 produced in HEK293T cell. |
Conjugate |
Unconjugated |
Form |
lyophilized |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 8% trehalose |
Contains |
no preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation (PubMed:9582349, PubMed:17368478, PubMed:25624349). Acts as a repressor of translation initiation (PubMed:22751931). In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: eIF-4E type 2; eIF4E-like cap-binding protein; eIF4E-like protein 4E-LP; eukar; Eukaryotic translation initiation factor 4E homologous protein; Eukaryotic translation initiation factor 4E type 2; Eukaryotic translation initiation factor 4E-like 3; h4EHP; mRNA cap-binding protein 4EHP; mRNA cap-binding protein type 3
Gene Aliases: 4E-LP; 4EHP; EIF4E2; EIF4EL3; IF4e
UniProt ID: (Human) O60573
Entrez Gene ID: (Human) 9470
Molecular Function:
translation factor
translation initiation factor
translational protein
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support