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ENOX2 Antibody (MA5-51316) in WB
Western blot analysis of ENOX2 in extracts of MCF7 cells. Samples were incubated with ENOX2 Recombinant monoclonal antibody (Product # MA5-51316) using a dilution of 1:2,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:200,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Product Details
MA5-51316
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5P7H2
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 411-610 of human ENOX2 (NP_872114.1).
Conjugate
Form
Liquid
Concentration
0.51 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3092443
Product Specific Information
Immunogen sequence: AEALKEENDS LRWQLDAYRN EVELLKQEQG KVHREDDPNK EQQLKLLQQA LQGMQQHLLK VQEEYKKKEA ELEKLKDDKL QVEKMLENLK EKESCASRLC ASNQDSEYPL EKTMNSSPIK SEREALLVGI ISTFLHVHPF GASIEYICSY LHRLDNKICT SDVECLMGRL QHTFKQEMTG VGASLEKRWK FCGFEGLKLT
Target Information
This gene is a tumor-specific member of the ECTO-NOX family of genes that encode cell surface NADH oxidases. The encoded protein has two enzymatic activities: catalysis of hydroquinone or NADH oxidation, and protein disulfide interchange. The protein also displays prion-like properties. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APK1 antigen; Cytosolic ovarian carcinoma antigen 1; Ecto-NOX disulfide-thiol exchanger 2; tNOX; Tumor-associated hydroquinone oxidase
Gene Aliases: APK1; COVA1; ENOX2; RGD1563643; tNOX
UniProt ID: (Human) Q16206
Entrez Gene ID: (Human) 10495, (Rat) 302817
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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